THE COMBINATION PROCESS FOR PREPARATIVE SEPARATION AND PURIFICATION OF PACLITAXEL AND 10-DEACETYLBACCATIN III USING DIAION® HP-20 FOLLOWED BY HYDROPHILIC INTERACTION BASED SOLID PHASE EXTRACTION

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SHIRSHEKAN MAHSA; REZADOOST HASSAN; JAVANBAKHT MEHRAN; GHASSEMPOUR ALIREZA

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Journal Paper

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Journal: Iranian Journal of Pharmaceutical Research Year:2017 | Volume:16 | Issue:4 Page(s): 1396-1404

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Title

THE COMBINATION PROCESS FOR PREPARATIVE SEPARATION AND PURIFICATION OF PACLITAXEL AND 10-DEACETYLBACCATIN III USING DIAION® HP-20 FOLLOWED BY HYDROPHILIC INTERACTION BASED SOLID PHASE EXTRACTION

Author(s)

SHIRSHEKAN MAHSA | REZADOOST HASSAN | JAVANBAKHT MEHRAN | GHASSEMPOUR ALIREZA | Issue Writer Certificate

Keywords

PACLITAXEL

10-DEACETYLBACCATIN III

DIAION® HP-20

HYDROPHILIC INTERACTION

SEMI- PREPARATIVE HPLC

Abstract

There is no other naturally occurring defense agent against cancer that has a stronger effect than PACLITAXEL, commonly known under the brand name of TaxolÒ. The major drawback for the more widespread use of PACLITAXEL and its precious precursor, 10-DEACETYLBACCATIN III (10-DAB III), is that they require large- scale extraction from different parts of yew trees (Taxus species), cell cultures, taxane- producing endophytic fungi, and Corylus species. In our previous work, a novel online two- dimensional heart- cut liquid chromatography process using HYDROPHILIC INTERACTION/ reversed-phase chromatography was used to introduce a semi-preparative treatment for the separation of polar (10-deacetylbaccatin III) and non-polar (PACLITAXEL) taxanes from Taxus baccata L. In this work, a combination of the absorbent (DiaionÒ HP- 20) and a silica based solid phase extraction is utilized as a new, efficient, and cost effective method for large-scale production of taxanes. This process avoids the technical problem of two- dimensional preparative liquid chromatography. The first stage of the process involves discarding co-extractive polar compounds including chlorophylls and pigments using a non-polar synthetic hydrophobic absorbent, DiaionÒ HP-20. Extract was then loaded on to a silica based HYDROPHILIC INTERACTION solid phase extraction (silica 40-60 micron). Taxanes was eluted using a mixture of water and methanol at the optimized ratio of 70: 30. Finally, the fraction containing taxanes was applied to semi-preparative reversed phase HPLC. The results revealed that using this procedure, PACLITAXEL and 10-DAB III could be obtained at 8 and 3 times more, respectively than by the traditional method of extraction.

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APA: Copy

SHIRSHEKAN, MAHSA, REZADOOST, HASSAN, JAVANBAKHT, MEHRAN, & GHASSEMPOUR, ALIREZA. (2017). THE COMBINATION PROCESS FOR PREPARATIVE SEPARATION AND PURIFICATION OF PACLITAXEL AND 10-DEACETYLBACCATIN III USING DIAION^® HP-20 FOLLOWED BY HYDROPHILIC INTERACTION BASED SOLID PHASE EXTRACTION. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), 16(4), 1396-1404. SID. https://sid.ir/paper/288714/en

Vancouver: Copy

SHIRSHEKAN MAHSA, REZADOOST HASSAN, JAVANBAKHT MEHRAN, GHASSEMPOUR ALIREZA. THE COMBINATION PROCESS FOR PREPARATIVE SEPARATION AND PURIFICATION OF PACLITAXEL AND 10-DEACETYLBACCATIN III USING DIAION^® HP-20 FOLLOWED BY HYDROPHILIC INTERACTION BASED SOLID PHASE EXTRACTION. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR)[Internet]. 2017;16(4):1396-1404. Available from: https://sid.ir/paper/288714/en

IEEE: Copy

MAHSA SHIRSHEKAN, HASSAN REZADOOST, MEHRAN JAVANBAKHT, and ALIREZA GHASSEMPOUR, “THE COMBINATION PROCESS FOR PREPARATIVE SEPARATION AND PURIFICATION OF PACLITAXEL AND 10-DEACETYLBACCATIN III USING DIAION^® HP-20 FOLLOWED BY HYDROPHILIC INTERACTION BASED SOLID PHASE EXTRACTION,” IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), vol. 16, no. 4, pp. 1396–1404, 2017, [Online]. Available: https://sid.ir/paper/288714/en

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