SINGLE STEP PURIFICATION OF NOVEL THERMOSTABLE AND CHELATOR RESISTANTAMYLASE FROM BACILLUS LICHENIFORMIS RM44 BY AFFINITY CHROMATOGRAPHY

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SIDDIQUI AYESHA; KAMAL MUSTAFA; Ayatollahi Seyed Abdulmajid; ALI MOHSIN; AHMED MANSOOR

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Journal: Iranian Journal of Pharmaceutical Research Year:2017 | Volume:16 | Issue:3 Page(s): 1141-1146

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Title

SINGLE STEP PURIFICATION OF NOVEL THERMOSTABLE AND CHELATOR RESISTANTAMYLASE FROM BACILLUS LICHENIFORMIS RM44 BY AFFINITY CHROMATOGRAPHY

Author(s)

Keywords

AMYLASE

AFFINITY CHROMATOGRAPHY

THERMOSTABLE

CHELATOR RESISTANT

SDSRESISTANT

Abstract

Bacillus licheniformis RM44 was isolated from hot spring near Karachi and screened forthe production of extracellular AMYLASE Amy RM44. Amy RM44 was purified to homogeneityon a single step by AFFINITY CHROMATOGRAPHY using insoluble corn starch. The molecular weightof Amy RM44 was estimated to be 66 kDa by SDS– PAGE and zymographic analysis. Nine foldpurification was achieved with the specific activity of 870 U/mg that provides the total yieldof the enzyme up to 31%. Studies on purified AmyRM44 characterization revealed that theoptimum temperature of enzyme was 100 º C. Amy RM44 was proved to be highly THERMOSTABLEas it retained 50% activity after 2 h at 100 º C. Amy RM44 was stable over wide range of pHwith optimum activity at pH 5. Enzyme activity was not significantly inhibited by SDS andEDTA. Amy RM44 also exhibited its activity towards various carbohydrates such as dextrin, pullulan, α-cyclodextrin, β-cyclodextrin, and γ-cyclodextrin.

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APA: Copy

SIDDIQUI, AYESHA, KAMAL, MUSTAFA, Ayatollahi, Seyed Abdulmajid, ALI, MOHSIN, & AHMED, MANSOOR. (2017). SINGLE STEP PURIFICATION OF NOVEL THERMOSTABLE AND CHELATOR RESISTANTAMYLASE FROM BACILLUS LICHENIFORMIS RM44 BY AFFINITY CHROMATOGRAPHY. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), 16(3), 1141-1146. SID. https://sid.ir/paper/288995/en

Vancouver: Copy

SIDDIQUI AYESHA, KAMAL MUSTAFA, Ayatollahi Seyed Abdulmajid, ALI MOHSIN, AHMED MANSOOR. SINGLE STEP PURIFICATION OF NOVEL THERMOSTABLE AND CHELATOR RESISTANTAMYLASE FROM BACILLUS LICHENIFORMIS RM44 BY AFFINITY CHROMATOGRAPHY. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR)[Internet]. 2017;16(3):1141-1146. Available from: https://sid.ir/paper/288995/en

IEEE: Copy

AYESHA SIDDIQUI, MUSTAFA KAMAL, Seyed Abdulmajid Ayatollahi, MOHSIN ALI, and MANSOOR AHMED, “SINGLE STEP PURIFICATION OF NOVEL THERMOSTABLE AND CHELATOR RESISTANTAMYLASE FROM BACILLUS LICHENIFORMIS RM44 BY AFFINITY CHROMATOGRAPHY,” IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), vol. 16, no. 3, pp. 1141–1146, 2017, [Online]. Available: https://sid.ir/paper/288995/en

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