Noscapine Inhibiting the Growth and Angiogenesis of Human Eutopic Endometrium of Endometriosis Patients through Expression of Apoptotic Genes and Nitric Oxide Reduction in Three-Dimensional Culture Model

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KHAZAEI MOHAMMAD RASOOL; NASR ESFAHANI MOHAMMAD HOSSEIN; CHOBSAZ FARZANEH; KHAZAEI MOZAFAR

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Journal: Iranian Journal of Pharmaceutical Research Year:2019 | Volume:18 | Issue:2 Page(s): 836-845

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Title

Noscapine Inhibiting the Growth and Angiogenesis of Human Eutopic Endometrium of Endometriosis Patients through Expression of Apoptotic Genes and Nitric Oxide Reduction in Three-Dimensional Culture Model

Author(s)

KHAZAEI MOHAMMAD RASOOL | NASR ESFAHANI MOHAMMAD HOSSEIN | CHOBSAZ FARZANEH | KHAZAEI MOZAFAR | Issue Writer Certificate

Keywords

Endometriosis

Noscapine

Apoptosis

Nitric oxide

Sirtuin

Abstract

Noscapine is a natural alkaloid with anti-angiogenesis activities. The aim of the present study was to examine the effect of Noscapine on eutopic endometrium of Endometriosis patients (EEE) and normal endometrium (NE) in a three-dimensional (3D) culture model. In this experimental in-vitro study, EEE (n = 8) and NE (n = 8) biopsies were taken from 16 reproductive aged women. The biopsies were cleared from blood and mucus. Each biopsy was cut into small fragments (1 × 1 mm) in a sterile condition. For 3D culture, the endometrial fragments were put between two layers of fibrin jell made of fibrinogen solution [3 mg/mL in Medium199 (M199) + thrombin]. Twenty-four wells of culture dish was divided into 5 groups for each biopsy: the control wells were treated with M199 containing 5% fetal bovine serum (FBS) while, the test wells were exposed to the same media containing one of the Noscapine doses (10, 50, 100, and 200 μ M). The expression of apoptotic genes, growth score, angiogenesis, and Nitric oxide (NO) secretion were evaluated. The mean of growth score of groups exposed to 0, 10, 50, 100, and 200 μ M were 2. 2 ± 0. 55, 1. 7 ± 0. 45, 1. 44 ± 0. 27, 0. 29 ± 0. 1, and 0. 1 ± 0. 08 in EEE, and also, 2. 11 ± 0. 6, 1. 65 ± 0. 5, 0. 79 ± 0. 41, 0. 18 ± 0. 1, and 0. 1 ± 0. 1 in NE, respectively, and the difference between the groups was significant (P < 0. 05). The expression of apoptotic genes significantly increased while, the levels of Bcl-2 and Sirt1 reduced (P = 0. 004). NO secretion reduced significantly (P < 0. 05) in both EEE and NE groups. In conclusion, higher doses of Noscapine showed inhibitory effect on growth and angiogenesis of EEE and NE.

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APA: Copy

KHAZAEI, MOHAMMAD RASOOL, NASR ESFAHANI, MOHAMMAD HOSSEIN, CHOBSAZ, FARZANEH, & KHAZAEI, MOZAFAR. (2019). Noscapine Inhibiting the Growth and Angiogenesis of Human Eutopic Endometrium of Endometriosis Patients through Expression of Apoptotic Genes and Nitric Oxide Reduction in Three-Dimensional Culture Model. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), 18(2), 836-845. SID. https://sid.ir/paper/289104/en

Vancouver: Copy

KHAZAEI MOHAMMAD RASOOL, NASR ESFAHANI MOHAMMAD HOSSEIN, CHOBSAZ FARZANEH, KHAZAEI MOZAFAR. Noscapine Inhibiting the Growth and Angiogenesis of Human Eutopic Endometrium of Endometriosis Patients through Expression of Apoptotic Genes and Nitric Oxide Reduction in Three-Dimensional Culture Model. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR)[Internet]. 2019;18(2):836-845. Available from: https://sid.ir/paper/289104/en

IEEE: Copy

MOHAMMAD RASOOL KHAZAEI, MOHAMMAD HOSSEIN NASR ESFAHANI, FARZANEH CHOBSAZ, and MOZAFAR KHAZAEI, “Noscapine Inhibiting the Growth and Angiogenesis of Human Eutopic Endometrium of Endometriosis Patients through Expression of Apoptotic Genes and Nitric Oxide Reduction in Three-Dimensional Culture Model,” IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), vol. 18, no. 2, pp. 836–845, 2019, [Online]. Available: https://sid.ir/paper/289104/en

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