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Information Journal Paper

Title

Synthesis of a Peptide Derivative of MicrocinJ25 and Evaluation of Antibacterial and Biological Activities

Pages

  1264-1276

Abstract

 MicrocinJ25 (MccJ25) is a small ribosomally synthesized antimicrobial peptide that is produced by Enterobacteriacea family especially E. coli. The present study focuses on preparation and evaluation of in-vitro antimicrobial and biological properties of a new peptide derived from MccJ25. We prepared a MccJ25-derived peptide containing 14 amino acids and a single intra-molecular disulfide bond according to solid phase Synthesis strategy. The purified peptide was characterized by Liquid chromatography-mass spectrometry (LC-MS) and Fourier Transform Infrared (FTIR) spectroscopy. 96-well microdilution plate assay was exerted for determination of minimum inhibitory concentration (MIC) of peptide against different bacterial strains. Cytotoxicity of the peptide derivative on HT-29 cell line assayed using MTT test. The final peptide successfully was prepared with purity more than 99. 8% as determined by analytical HPLC. The evaluation of antibacterial activity of the peptide against Gram-positive and Gram-negative bacteria revealed that the peptide was very effective against E. coli 35218 with minimum inhibitory concentration (MIC) at dose 3. 9 μ M. The hemolytic activity toward human erythrocytes was very minimal below 0. 3%. The cell viability percentage of HT-29 cell line after 24 h of contact with the peptide was more than 83%. The high sensitivity of E. coli strain to this new peptide derived from MccJ25 and through minimal toxicity to cancerous cell, suggesting that above synthesized peptide could be considered as a bioactive compound for further investigations.

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    • Cite

    APA: Copy

    Mazaheri Tehrani, Maryam, ERFANI, MOSTAFA, AMIRMOZAFARI, NOUR, & NEJADSATTARI, TAHER. (2019). Synthesis of a Peptide Derivative of MicrocinJ25 and Evaluation of Antibacterial and Biological Activities. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), 18(3), 1264-1276. SID. https://sid.ir/paper/289139/en

    Vancouver: Copy

    Mazaheri Tehrani Maryam, ERFANI MOSTAFA, AMIRMOZAFARI NOUR, NEJADSATTARI TAHER. Synthesis of a Peptide Derivative of MicrocinJ25 and Evaluation of Antibacterial and Biological Activities. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR)[Internet]. 2019;18(3):1264-1276. Available from: https://sid.ir/paper/289139/en

    IEEE: Copy

    Maryam Mazaheri Tehrani, MOSTAFA ERFANI, NOUR AMIRMOZAFARI, and TAHER NEJADSATTARI, “Synthesis of a Peptide Derivative of MicrocinJ25 and Evaluation of Antibacterial and Biological Activities,” IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), vol. 18, no. 3, pp. 1264–1276, 2019, [Online]. Available: https://sid.ir/paper/289139/en

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