Physical Separation of Amphiprotic-Polar AproticS for Simultaneous Extraction and Clean-up of Clomiphene from Plasma before Liquid Chromatographic Analysis

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AHMADI FARHAD; Rahmani Nilofar

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Journal: Iranian Journal of Pharmaceutical Research Year:2019 | Volume:18 | Issue:3 Page(s): 1212-1223

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Information Journal Paper

Title

Physical Separation of Amphiprotic-Polar AproticS for Simultaneous Extraction and Clean-up of Clomiphene from Plasma before Liquid Chromatographic Analysis

Author(s)

AHMADI FARHAD | Rahmani Nilofar | Issue Writer Certificate

Keywords

Clomiphene citrate

Two phase freezing method

Liquid-liquid extraction

Doping control

Plasma extraction

Abstract

An efficient and quantitative two phase freezing (TPF) method coupled with high performance liquid chromatography and UV-Vis detector was developed for the extraction, clean up, and determination of Clomiphene citrate (CLC) in plasma samples. The separation of two miscible solvents by TPF method permits that the CLC was efficiently removed from proteins and transferred into the relative aprotic dipolar organic phase and in consequence, gave a higher recovery. The TPF method was compared to conventional Liquid-liquid extraction and it gave more clean solution with better reproducibility. Linear range, limit of detection, and limit of quantification for CLC in plasma were obtained in the range of 0. 06-18, 0. 02, and 0. 06 μ g mL-1, respectively. The intraday and interday reproducibility for concentration of 1. 0 μ g mL-1 (%RSD) were 3. 2% and 4. 6%, respectively. In addition, the trueness, ruggedness, and reality of TPF were assessment. Finally, several real plasma samples were successfully analyzed using the developed method.

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APA: Copy

AHMADI, FARHAD, & Rahmani, Nilofar. (2019). Physical Separation of Amphiprotic-Polar AproticS for Simultaneous Extraction and Clean-up of Clomiphene from Plasma before Liquid Chromatographic Analysis. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), 18(3), 1212-1223. SID. https://sid.ir/paper/289143/en

Vancouver: Copy

AHMADI FARHAD, Rahmani Nilofar. Physical Separation of Amphiprotic-Polar AproticS for Simultaneous Extraction and Clean-up of Clomiphene from Plasma before Liquid Chromatographic Analysis. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR)[Internet]. 2019;18(3):1212-1223. Available from: https://sid.ir/paper/289143/en

IEEE: Copy

FARHAD AHMADI, and Nilofar Rahmani, “Physical Separation of Amphiprotic-Polar AproticS for Simultaneous Extraction and Clean-up of Clomiphene from Plasma before Liquid Chromatographic Analysis,” IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), vol. 18, no. 3, pp. 1212–1223, 2019, [Online]. Available: https://sid.ir/paper/289143/en

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