Mitochondrial Complex I Is an Essential Player in LPS-Induced Preconditioning in Differentiated PC12 Cells

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MANOUCHEHRI NASIM; KHODAGHOLI FARIBA; DARGAHI LEILA; AHMADIANI ABOLHASSAN

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Journal Paper

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Journal: Iranian Journal of Pharmaceutical Research Year:2019 | Volume:18 | Issue:3 Page(s): 1445-1455

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Title

Mitochondrial Complex I Is an Essential Player in LPS-Induced Preconditioning in Differentiated PC12 Cells

Author(s)

MANOUCHEHRI NASIM | KHODAGHOLI FARIBA | DARGAHI LEILA | AHMADIANI ABOLHASSAN | Issue Writer Certificate

Keywords

Mitochondrial complex I

Preconditioning

Neuroinflammation

Neuroprotection

Mitochondria

Abstract

Preconditioning (PC) as a protective strategy against noxious insults can decline cell death and apoptosis. It has been approved that Mitochondria play a key role in PC mechanism. The critical role of complex I (CI) in oxidative phosphorylation machinery and intracellular ROS production, particularly in the brain, accentuates its possible role in PC-induced Neuroprotection. Here, differentiated PC12 cells were preconditioned with ultra-low dose LPS (ULD, 3 μ g/mL) prior to exposure to high concentration of LPS (HD, 750 μ g/mL). Our results showed that HD LPS treatment reduces cell viability and CI activity, and intensifies expression of cleaved caspase 3 compared to the control group. Intriguingly, PC induction resulted in enhancement of cell viability and CI activity and reduction of caspase3 cleavage compared to HD LPS group. In order to explore the role of CI in PC, we combined the ULD LPS with rotenone, a CI inhibitor. Following rotenone administration, cell viability significantly reduced while caspase3 cleavage increased compared to PC induction group. Taken together, cell survival and reduction of apoptosis followed by PC can be at least partially attributed to the preservation of Mitochondrial CI function.

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APA: Copy

MANOUCHEHRI, NASIM, KHODAGHOLI, FARIBA, DARGAHI, LEILA, & AHMADIANI, ABOLHASSAN. (2019). Mitochondrial Complex I Is an Essential Player in LPS-Induced Preconditioning in Differentiated PC12 Cells. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), 18(3), 1445-1455. SID. https://sid.ir/paper/289167/en

Vancouver: Copy

MANOUCHEHRI NASIM, KHODAGHOLI FARIBA, DARGAHI LEILA, AHMADIANI ABOLHASSAN. Mitochondrial Complex I Is an Essential Player in LPS-Induced Preconditioning in Differentiated PC12 Cells. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR)[Internet]. 2019;18(3):1445-1455. Available from: https://sid.ir/paper/289167/en

IEEE: Copy

NASIM MANOUCHEHRI, FARIBA KHODAGHOLI, LEILA DARGAHI, and ABOLHASSAN AHMADIANI, “Mitochondrial Complex I Is an Essential Player in LPS-Induced Preconditioning in Differentiated PC12 Cells,” IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), vol. 18, no. 3, pp. 1445–1455, 2019, [Online]. Available: https://sid.ir/paper/289167/en

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