OPTIMIZATION OF IN VITRO CULTURE OF MILK THISTLE (SILYBUM MARIANUM) AND ANALYSIS OF SOMACLONAL VARIATION BY RAPD MARKERS

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POURJABAR A.; MOHAMMADI S.A.; KHOSROSHAHLI M.; MOTALEBI A.R.; ZIAI S.A.

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Journal: JOURNAL OF AGRICULTURAL KNOWLEDGE Year:2009 | Volume:19 | Issue:2 Page(s): 95-108

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Title

OPTIMIZATION OF IN VITRO CULTURE OF MILK THISTLE (SILYBUM MARIANUM) AND ANALYSIS OF SOMACLONAL VARIATION BY RAPD MARKERS

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Keywords

KINETINQ3

MILK THISTLEQ3

SOMACLONAL VARIATIONQ3

TISSUE CULTUREQ3

2-4 DQ3

Abstract

Milk thistle (Silybum marianum) is one of the most important medicinal plants and has found special place in pharmacy industries. Irregular harvest of this plant from natural habitats results in the destruction of its genetic resources. In order to optimize in vitro culture of MILK THISTLE, this study was carried out in a factorial experiment based on completely randomized design with four replications, two genotypes (Budakalszi from Hungry and Moghan ecotype) and cotyledon and hypocotyle explants in MS medium supplemented with four KINETIN and 2-4 D combinations. Seeds were germinated in MS medium without hormones under 26oC and darkness conditions. After callus induction, the calli were subcultured four times into the fresh media. Analysis of variance revealed that in all of the subcultures, Budakalszi cultivar was superior to Moghan ecotype with respect to callus fresh weight and diameter. Callus fresh weight and diameter were also higher in the calli obtained from cotyledon compared with the hypocotyls explant. With the increase in hormone levels, callus weight and diameter were increased. In all of the four subcultures, maximum callus growth was observed in the medium supplemented with 2 mg/l 2-4 D and 2 mg/l KINETIN. Analysis of SOMACLONAL VARIATION in the calli obtained from different culture conditions and subcultures using RAPD markers revealed different banding patterns among samples from explants of the two genotypes in different culture and subculture conditions.

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APA: Copy

POURJABAR, A., MOHAMMADI, S.A., KHOSROSHAHLI, M., MOTALEBI, A.R., & ZIAI, S.A.. (2009). OPTIMIZATION OF IN VITRO CULTURE OF MILK THISTLE (SILYBUM MARIANUM) AND ANALYSIS OF SOMACLONAL VARIATION BY RAPD MARKERS. JOURNAL OF AGRICULTURAL KNOWLEDGE, 19(2), 95-108. SID. https://sid.ir/paper/29081/en

Vancouver: Copy

POURJABAR A., MOHAMMADI S.A., KHOSROSHAHLI M., MOTALEBI A.R., ZIAI S.A.. OPTIMIZATION OF IN VITRO CULTURE OF MILK THISTLE (SILYBUM MARIANUM) AND ANALYSIS OF SOMACLONAL VARIATION BY RAPD MARKERS. JOURNAL OF AGRICULTURAL KNOWLEDGE[Internet]. 2009;19(2):95-108. Available from: https://sid.ir/paper/29081/en

IEEE: Copy

A. POURJABAR, S.A. MOHAMMADI, M. KHOSROSHAHLI, A.R. MOTALEBI, and S.A. ZIAI, “OPTIMIZATION OF IN VITRO CULTURE OF MILK THISTLE (SILYBUM MARIANUM) AND ANALYSIS OF SOMACLONAL VARIATION BY RAPD MARKERS,” JOURNAL OF AGRICULTURAL KNOWLEDGE, vol. 19, no. 2, pp. 95–108, 2009, [Online]. Available: https://sid.ir/paper/29081/en

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