IDENTIFICATION OF HITHERTO UNDEFINED B-CELL EPITOPES BY ANTIBODIES IN THE
SERA OF VITILIGO PATIENTS USING PHAGE-DISPLAY PEPTIDE LIBRARY

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JADALI Z.; ESLAMI M.B.; MAHMOUDI M.; SANATI M.H.; MAGHSOUDI N.; MANSOURI PARVIN; ARBABI GHAHROUDI M.

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Journal Paper

Paper Information

Journal: Iranian Journal of Allergy, Asthma and Immunology Year:2003 | Volume:2 | Issue:4 Page(s): 197-201

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Information Journal Paper

Title

IDENTIFICATION OF HITHERTO UNDEFINED B-CELL EPITOPES BY ANTIBODIES IN THE SERA OF VITILIGO PATIENTS USING PHAGE-DISPLAY PEPTIDE LIBRARY

Author(s)

Keywords

B-CELL EPITOPE

RANDOM PEPTIDE LIBRARY

SELF-ANTIGEN

VITILIGO

Abstract

A random 12 mers phage library was used to screen a pool of immunoglobulin fractions obtained from VITILIGO patients. Subsequent to panning experiments, a panel of affinity selected phage from VITILIGO patients was obtained. This panel was tested using an ELISA for their reactivity with pooled sera from patients and normal controls. Among the 16 randomly selected clones, two of clones showed distinct positive reactivity with the patient’s sera compared with controls. The peptides displayed by these phages expressed the following amino acid sequences: SHMPLANQYQWA and NHVQAWEQFWDS. Thus, screening with phagedisplayed RANDOM PEPTIDE LIBRARY of VITILIGO sera can reveal peptide sequences that mimic VITILIGO-related SELF-ANTIGEN.

Cites

THE AUTOIMMUNE DISEASES MANIFESTED BY PRODUCTION OF AUTOANTIBODIES: THE AUTOANTIGENS IDENTIFIED BY RANDOM PEPTIDE LIBRARY

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APA: Copy

JADALI, Z., ESLAMI, M.B., MAHMOUDI, M., SANATI, M.H., MAGHSOUDI, N., MANSOURI, PARVIN, & ARBABI GHAHROUDI, M.. (2003). IDENTIFICATION OF HITHERTO UNDEFINED B-CELL EPITOPES BY ANTIBODIES IN THE SERA OF VITILIGO PATIENTS USING PHAGE-DISPLAY PEPTIDE LIBRARY . IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI), 2(4), 197-201. SID. https://sid.ir/paper/291044/en

Vancouver: Copy

JADALI Z., ESLAMI M.B., MAHMOUDI M., SANATI M.H., MAGHSOUDI N., MANSOURI PARVIN, ARBABI GHAHROUDI M.. IDENTIFICATION OF HITHERTO UNDEFINED B-CELL EPITOPES BY ANTIBODIES IN THE SERA OF VITILIGO PATIENTS USING PHAGE-DISPLAY PEPTIDE LIBRARY . IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI)[Internet]. 2003;2(4):197-201. Available from: https://sid.ir/paper/291044/en

IEEE: Copy

Z. JADALI, M.B. ESLAMI, M. MAHMOUDI, M.H. SANATI, N. MAGHSOUDI, PARVIN MANSOURI, and M. ARBABI GHAHROUDI, “IDENTIFICATION OF HITHERTO UNDEFINED B-CELL EPITOPES BY ANTIBODIES IN THE SERA OF VITILIGO PATIENTS USING PHAGE-DISPLAY PEPTIDE LIBRARY ,” IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI), vol. 2, no. 4, pp. 197–201, 2003, [Online]. Available: https://sid.ir/paper/291044/en

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