COMPARISON OF ELISA AND IFA FOR ESTIMATION OF ANTIBODY LEVELS OF
CATTLE TO THEILERIA ANNULATA VACCINE

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GOLCHINFAR F.; MADANI R.; HASHEMI FESHARAKI R.; ESMAILNYA K.

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Journal Paper

Paper Information

Journal: Iranian Journal of Allergy, Asthma and Immunology Year:2003 | Volume:2 | Issue:2 Page(s): 101-106

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Title

COMPARISON OF ELISA AND IFA FOR ESTIMATION OF ANTIBODY LEVELS OF CATTLE TO THEILERIA ANNULATA VACCINE

Author(s)

GOLCHINFAR F. | MADANI R. | HASHEMI FESHARAKI R. | ESMAILNYA K. | Issue Writer Certificate

Keywords

THEILERIA ANNULATA

ELISA

IPA

VACCINE.

Abstract

Bovine tropical Theileriosis caused by THEILERIA ANNULATA is an economically important disease of cattle. An enzyme linked immunosorbent assay (ELISA) was used to detelmine antibody levels in vaccinated and unvaccinated cattle, using cellular schizont as antigen and its results were compared with immunofluorescent assay (IFA). For this test 126 sera collected (105 vaccinated, 31 not vaccinated) from cows and assayed with ELISA which among them 104 sera were positive and 32 sera were negative. Same sample assayed with IFA in which 99 were positive sera and 37 were negative sera. Thereby the sensitivity and specificity of this ELISA on comparsion with IFA were 95.5% and 66.6% respectively. This study revealed that ELISA could be successfully used for both differentiating vaccinated and not vaccinated cattle and obtaining the titer of vaccinated cattle.

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APA: Copy

GOLCHINFAR, F., MADANI, R., HASHEMI FESHARAKI, R., & ESMAILNYA, K.. (2003). COMPARISON OF ELISA AND IFA FOR ESTIMATION OF ANTIBODY LEVELS OF CATTLE TO THEILERIA ANNULATA VACCINE. IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI), 2(2), 101-106. SID. https://sid.ir/paper/291069/en

Vancouver: Copy

GOLCHINFAR F., MADANI R., HASHEMI FESHARAKI R., ESMAILNYA K.. COMPARISON OF ELISA AND IFA FOR ESTIMATION OF ANTIBODY LEVELS OF CATTLE TO THEILERIA ANNULATA VACCINE. IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI)[Internet]. 2003;2(2):101-106. Available from: https://sid.ir/paper/291069/en

IEEE: Copy

F. GOLCHINFAR, R. MADANI, R. HASHEMI FESHARAKI, and K. ESMAILNYA, “COMPARISON OF ELISA AND IFA FOR ESTIMATION OF ANTIBODY LEVELS OF CATTLE TO THEILERIA ANNULATA VACCINE,” IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI), vol. 2, no. 2, pp. 101–106, 2003, [Online]. Available: https://sid.ir/paper/291069/en

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