EFFECT OF ALL-TRANS RETINOIC ACID (ATRA) ON VIABILITY, PROLIFERATION, ACTIVATION AND LINEAGE-SPECIFIC TRANSCRIPTION FACTORS OF CD4+ T CELLS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

BIDAD KATAYOON; SALEHI EISA; ORAEI MONA; SABOOR YARAGHI ALI AKBAR; NICKNAM MOHAMMAD HOSSEIN

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Allergy, Asthma and Immunology Year:2011 | Volume:10 | Issue:4 Page(s): 243-249

Download Full-Text

View:

308

Download:

160

Cites:

Information Journal Paper

Title

EFFECT OF ALL-TRANS RETINOIC ACID (ATRA) ON VIABILITY, PROLIFERATION, ACTIVATION AND LINEAGE-SPECIFIC TRANSCRIPTION FACTORS OF CD4+ T CELLS

Author(s)

Keywords

ATRA

CD4+ T CELLS

FOXP3

RORγT

TRANSCRIPTION FACTORS

T-BET

Abstract

All-trans retinoic acid (ATRA), as an active metabolite of vitamin A, has been shown to affect immune cells. This study was performed to evaluate the effect of ATRA on viability, proliferation, activation and lineage-specific TRANSCRIPTION FACTORS of CD4+T cells. CD4+T cells were separated from heparinized blood of healthy donors and were cultured in conditions, some with, some without ATRA.Viability was assessed by PI flowcytometry and proliferation was measured by MTT assay. CD69 expression was determined by flowcytometry as a measure of cell activation.Lineage-specific TRANSCRIPTION FACTORS (FOXP3, RORgt and T-BET) were examined by intracellular staining and flowcytometry. High doses of ATRA (0.1-1 mM) caused extensive cell death in both PBMCs and CD4+T cells. Doses of ATRA equal to or lower than 10 mM did not adversely affect cell viability and proliferation in comparison to culture medium without ATRA.Doses of ATRA between 10 mM and 1nM significantly increased cell activation when compared to culture medium without ATRA. ATRA could increase FOXP3+and also FOXP3+RORgt+T cells while it decreased RORgt+and T-BET+T cells. This study showed that doses of ATRA up to 10 mM are safe when using with CD4+T cells in terms of cell viability, proliferation and activation.We could also show that ATRA diverts the human immune response in neutral conditions (without adding polarizing cytokines) by increasing FOXP3+cells and decreasing RORgt+cells. ATRA could be regarded as a potential therapy in inflammatory conditions and autoimmunities.

Cites

No record.

References

No record.

Cite

APA: Copy

BIDAD, KATAYOON, SALEHI, EISA, ORAEI, MONA, SABOOR YARAGHI, ALI AKBAR, & NICKNAM, MOHAMMAD HOSSEIN. (2011). EFFECT OF ALL-TRANS RETINOIC ACID (ATRA) ON VIABILITY, PROLIFERATION, ACTIVATION AND LINEAGE-SPECIFIC TRANSCRIPTION FACTORS OF CD4+ T CELLS. IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI), 10(4), 243-249. SID. https://sid.ir/paper/291331/en

Vancouver: Copy

BIDAD KATAYOON, SALEHI EISA, ORAEI MONA, SABOOR YARAGHI ALI AKBAR, NICKNAM MOHAMMAD HOSSEIN. EFFECT OF ALL-TRANS RETINOIC ACID (ATRA) ON VIABILITY, PROLIFERATION, ACTIVATION AND LINEAGE-SPECIFIC TRANSCRIPTION FACTORS OF CD4+ T CELLS. IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI)[Internet]. 2011;10(4):243-249. Available from: https://sid.ir/paper/291331/en

IEEE: Copy

KATAYOON BIDAD, EISA SALEHI, MONA ORAEI, ALI AKBAR SABOOR YARAGHI, and MOHAMMAD HOSSEIN NICKNAM, “EFFECT OF ALL-TRANS RETINOIC ACID (ATRA) ON VIABILITY, PROLIFERATION, ACTIVATION AND LINEAGE-SPECIFIC TRANSCRIPTION FACTORS OF CD4+ T CELLS,” IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI), vol. 10, no. 4, pp. 243–249, 2011, [Online]. Available: https://sid.ir/paper/291331/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops