DEVELOPMENT OF A NEW IMMUNOCHROMATOGRAPHIC ASSAY USING GOLD NANOPARTICLES FOR SCREENING OF IGA DEFICIENCY

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GOUDARZI SAEID; AHMADI ANITA; FARHADI MOHAMMAD; KAMRAVA SEYED KAMRAN; SAGHAFI SHIVA; OMIDFAR KOBRA

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Journal Paper

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Journal: Iranian Journal of Allergy, Asthma and Immunology Year:2015 | Volume:14 | Issue:1 Page(s): 105-112

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Title

DEVELOPMENT OF A NEW IMMUNOCHROMATOGRAPHIC ASSAY USING GOLD NANOPARTICLES FOR SCREENING OF IGA DEFICIENCY

Author(s)

Keywords

IGA DEFICIENCY

IMMUNOGLOBULIN A

IMMUNOCHROMATOGRAPHY

GOLD NANOPARTICLE

SCREENING TEST

Abstract

A new competitive IMMUNOCHROMATOGRAPHY (ICG) strip test based on polyclonal antibody (pAb) conjugated with GOLD NANOPARTICLEs (NPs) was developed and its applications for primary screening of immunoglobulin (Ig) A in serum were evaluated. Nanocolloidal gold as the detection reagent, with an average particle diameter of 20 nm, was synthesized and labelled pAb.The antibody-nanocolloidal gold probe was applied on the conjugate pad, and human IgA was immobilized on a nitrocellulose membrane as the capture reagent to prepare the ICG strip test. It took only 10 minutes to accomplish a semi-quantitative detection of serum IgA in this assay.In the optimized investigational conditions, the ICG strip test could distinguish human serum IgA in the range from 1 to 270 ng/mL with a detection limit of 5 ng/mL.The reliability of testing procedures was examined by performing the ICG strip test with 11 serum samples and comparing the results with those obtained via enzyme-linked immunosorbent assay (ELISA). The ICG strip was sufficiently sensitive and accurate for a rapid screening of IgA in human serum.

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References

SELECTIVE IMMUNOGLOBULIN A DEFICIENCY IN IRANIAN BLOOD DONORS: PREVALENCE, LABORATORY AND CLINICAL FINDINGS

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APA: Copy

GOUDARZI, SAEID, AHMADI, ANITA, FARHADI, MOHAMMAD, KAMRAVA, SEYED KAMRAN, SAGHAFI, SHIVA, & OMIDFAR, KOBRA. (2015). DEVELOPMENT OF A NEW IMMUNOCHROMATOGRAPHIC ASSAY USING GOLD NANOPARTICLES FOR SCREENING OF IGA DEFICIENCY. IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI), 14(1), 105-112. SID. https://sid.ir/paper/291419/en

Vancouver: Copy

GOUDARZI SAEID, AHMADI ANITA, FARHADI MOHAMMAD, KAMRAVA SEYED KAMRAN, SAGHAFI SHIVA, OMIDFAR KOBRA. DEVELOPMENT OF A NEW IMMUNOCHROMATOGRAPHIC ASSAY USING GOLD NANOPARTICLES FOR SCREENING OF IGA DEFICIENCY. IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI)[Internet]. 2015;14(1):105-112. Available from: https://sid.ir/paper/291419/en

IEEE: Copy

SAEID GOUDARZI, ANITA AHMADI, MOHAMMAD FARHADI, SEYED KAMRAN KAMRAVA, SHIVA SAGHAFI, and KOBRA OMIDFAR, “DEVELOPMENT OF A NEW IMMUNOCHROMATOGRAPHIC ASSAY USING GOLD NANOPARTICLES FOR SCREENING OF IGA DEFICIENCY,” IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI), vol. 14, no. 1, pp. 105–112, 2015, [Online]. Available: https://sid.ir/paper/291419/en

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