CHARACTERIZATION OF TWO POLLEN ALLERGENS OF THE LONDON PLANE TREE IN SHANGHAI

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

CHEN ZHEN; YANG YI; CHEN XIAO DONG; WU ZHI LI; LI SHI FENG

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Allergy, Asthma and Immunology Year:2015 | Volume:14 | Issue:2 Page(s): 139-148

Download Full-Text

View:

404

Download:

143

Cites:

Information Journal Paper

Title

CHARACTERIZATION OF TWO POLLEN ALLERGENS OF THE LONDON PLANE TREE IN SHANGHAI

Author(s)

Keywords

LONDON PLANE TREE

MASS SPECTROMETRY

PLA A 1

PLA A 2

PLATANUS ACERIFOLIA

POLLEN ALLERGEN

Abstract

Platanus acerifolia, LONDON PLANE TREE, a significant source of airborne allergens, is widely grown in Shanghai and other cities in China. In recent decades, little has been known regarding the influence of the allergens on sensitizing the population in the Shanghai area. The aims of this study were to purify and characterize the two major allergens and to confirm the immunological activities of these POLLEN ALLERGENs in Shanghai.Crude extract was purified with a HiTrap SP column and a Sephedex G75 column. Immunodetection was performed with ELISA and immunoblotting. Following gel proteolytic digestion and MASS SPECTROMETRY, the tandem MS (MS/MS) peptide mass fingerprint was obtained and the MASCOT search engine was used to identify the peptide. The accession number of the interesting homologous data and all the sequence information was acquired by an internet database and the evolutionary trees were drawn with Mega 4.0 software.Two proteins with molecular weights of 43 kDa and 18 kDa were purified from P. acerifolia pollen extract. The purified proteins were identified as POLLEN ALLERGEN PLA A 1 and PLA A 2 via MASS SPECTROMETRY. The proteins have immunological activities with human IgE antibodies. According to the ELISA results, 12% (5/41) of the subjects were sensitive to PLA A 1 and 9% (4/41) were sensitive to PLA A 2.Pla a 1 and PLA A 2 are thus important allergens for patients with an allergic reaction to P. acerifolia pollen in Shanghai.

Cites

No record.

References

No record.

Cite

APA: Copy

CHEN, ZHEN, YANG, YI, CHEN, XIAO DONG, WU, ZHI LI, & LI, SHI FENG. (2015). CHARACTERIZATION OF TWO POLLEN ALLERGENS OF THE LONDON PLANE TREE IN SHANGHAI. IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI), 14(2), 139-148. SID. https://sid.ir/paper/291433/en

Vancouver: Copy

CHEN ZHEN, YANG YI, CHEN XIAO DONG, WU ZHI LI, LI SHI FENG. CHARACTERIZATION OF TWO POLLEN ALLERGENS OF THE LONDON PLANE TREE IN SHANGHAI. IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI)[Internet]. 2015;14(2):139-148. Available from: https://sid.ir/paper/291433/en

IEEE: Copy

ZHEN CHEN, YI YANG, XIAO DONG CHEN, ZHI LI WU, and SHI FENG LI, “CHARACTERIZATION OF TWO POLLEN ALLERGENS OF THE LONDON PLANE TREE IN SHANGHAI,” IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI), vol. 14, no. 2, pp. 139–148, 2015, [Online]. Available: https://sid.ir/paper/291433/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops