Expression of miR-15b-5p, miR-21-5p, and SMAD7 in Lung Tissue of Sulfur Mustard-exposedIndividuals with Long-term Pulmonary Complications

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SALIMI SAHAR; Noorbakhsh Farshid; FAGHIHZADEH SOGHRAT; GHAFFARPOUR SARA; GHAZANFARI TOOBA

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Journal: Iranian Journal of Allergy, Asthma and Immunology Year:2019 | Volume:18 | Issue:3 Page(s): 332-339

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Title

Expression of miR-15b-5p, miR-21-5p, and SMAD7 in Lung Tissue of Sulfur Mustard-exposedIndividuals with Long-term Pulmonary Complications

Author(s)

Keywords

miR-15b-5p

miR-21-5p

SMAD7

Sulfur mustard

Transforming growth factor β

Abstract

Sulfur mustard (SM)-exposed individuals develop late pulmonary complications, which are associated with chronic inflammation and fibrotic changes in the lung tissue. MicroRNAs are known to act as important regulators of inflammatory responses, including inflammation and fibrosis-related cytokine signaling. In this study, we investigated the expression miR-15b-5p and miR-21-5p, two regulators of TGF-β signaling, as well as their target molecule, SMAD7, in lung tissues from SM-exposed and control individuals. Total RNA was extracted from formalin-fixed paraffin-embedded (FFPE) lung tissue biopsies obtained during surgery from SM-exposed (n=20) or control (n=20) cases. Quality of the extracted RNA was evaluated by an Agilent Bioanalyzer and RNA was quantified using a NanoDrop. miR-21-5p, miR-15b-5p and SMAD7 expression levels were measured by realtime RT-PCR. miR-21-5p expression levels were significantly decreased (2. 7 fold) in the lung tissues from SM-exposed individuals compared with tissues obtained from the control group(p=0. 02). There were no significant differences in miR-15b-5p expression levels between the two groups(p=0. 94). Interestingly, SMAD7 expression levels were significantly higher (5. 8 fold) in SM-exposed individuals’ lung tissues compared with the control group(p=0. 045). Our data indicate that exposure to Sulfur mustard affects the expression of miR-21-5p as well as its target, SMAD7, in lung tissues many years after exposure. Considering the role of SMAD7 in the regulation of TGF-β signaling, these changes might point to a potential mechanism by which SM-exposure regulates inflammatory/fibrotic alterations in lung tissue.

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APA: Copy

SALIMI, SAHAR, Noorbakhsh, Farshid, FAGHIHZADEH, SOGHRAT, GHAFFARPOUR, SARA, & GHAZANFARI, TOOBA. (2019). Expression of miR-15b-5p, miR-21-5p, and SMAD7 in Lung Tissue of Sulfur Mustard-exposedIndividuals with Long-term Pulmonary Complications. IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI), 18(3), 332-339. SID. https://sid.ir/paper/291573/en

Vancouver: Copy

SALIMI SAHAR, Noorbakhsh Farshid, FAGHIHZADEH SOGHRAT, GHAFFARPOUR SARA, GHAZANFARI TOOBA. Expression of miR-15b-5p, miR-21-5p, and SMAD7 in Lung Tissue of Sulfur Mustard-exposedIndividuals with Long-term Pulmonary Complications. IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI)[Internet]. 2019;18(3):332-339. Available from: https://sid.ir/paper/291573/en

IEEE: Copy

SAHAR SALIMI, Farshid Noorbakhsh, SOGHRAT FAGHIHZADEH, SARA GHAFFARPOUR, and TOOBA GHAZANFARI, “Expression of miR-15b-5p, miR-21-5p, and SMAD7 in Lung Tissue of Sulfur Mustard-exposedIndividuals with Long-term Pulmonary Complications,” IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI), vol. 18, no. 3, pp. 332–339, 2019, [Online]. Available: https://sid.ir/paper/291573/en

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