ULTRAMICROSCOPIC STUDY OF TOXOPLASMA GONDII AND MECHANISM OF DYE TEST IN THE DIAGNOSIS OF TOXOPLASMOSIS

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GHANE M.; ASMAR M.; NOUHI ASHRAF ALSADAT; ESMAEILI RASTAGHI A.R.; SEYDIPOOR GH.H.

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Journal: HAKIM RESEARCH JOURNAL Year:2002 | Volume:5 | Issue:3 Page(s): 219-224

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Title

ULTRAMICROSCOPIC STUDY OF TOXOPLASMA GONDII AND MECHANISM OF DYE TEST IN THE DIAGNOSIS OF TOXOPLASMOSIS

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Keywords

TOXOPLASMOSISQ4

DYE TESTQ4

ELECTRON MICROSCOPYQ4

HUMORAL IMMUNITYQ4

Abstract

Toxoplasma gondii is an obligate intracellular protozoan parasite, capable of infecting all mammals, including human. Although TOXOPLASMOSIS is clinically asymptomatic in healthy individuals it can cause severe complication in pregnant women and immunocompromised patients.In this investigation, the ultrastructure of RH strain of Toxoplasma gondii and the effects of HUMORAL IMMUNITY against the organism on the basis of Sabin-Feldman DYE TEST have been studied using ELECTRON MICROSCOPY.It was observed that in the sera without specific antibody against Toxoplasma parasites, the cytoplasm of Toxoplasma was stained deeply, specially in the region of ribosomes. In this region, the rough endoplasmic reticulum was seen as dark lines. In those samples the morphology of the Toxoplasma had been altered and was seen swollen. Also it was found that when Toxoplasma exposed to antibody in the presence of accessory factor, its cytoplasm was completely unstained, compared to the deep blue staining of the cytoplasm of Toxoplasma in the mixtures with normal serum. In this mixtures the pellicle of Toxoplasma had been degenerated and there was an apparent extrusion of cytoplasmic contents. In fact, the accessory factor which acts in concert with antibody in human serum, is identical to classical complement pathway and so degenerates the pellicle of Toxoplasma. In addition, the ribosomes which normally present throughout the cytoplasm, lost their ability for staining in consequence of antibody effects, and thus treated organism failed to take up dye in the test. In these samples the nucleus was stained deeply.Considering the observations obtained in this study, it can be concluded that HUMORAL IMMUNITY can kill toxoplasma, but despite the ability of antibody to effectively kill extracellular Toxoplasma in vitro, the intracellular habitat of this protozoan in vivo is likely to protect it from the effects of circulating antibody.

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APA: Copy

GHANE, M., ASMAR, M., NOUHI, ASHRAF ALSADAT, ESMAEILI RASTAGHI, A.R., & SEYDIPOOR, GH.H.. (2002). ULTRAMICROSCOPIC STUDY OF TOXOPLASMA GONDII AND MECHANISM OF DYE TEST IN THE DIAGNOSIS OF TOXOPLASMOSIS. HAKIM RESEARCH JOURNAL, 5(3), 219-224. SID. https://sid.ir/paper/29175/en

Vancouver: Copy

GHANE M., ASMAR M., NOUHI ASHRAF ALSADAT, ESMAEILI RASTAGHI A.R., SEYDIPOOR GH.H.. ULTRAMICROSCOPIC STUDY OF TOXOPLASMA GONDII AND MECHANISM OF DYE TEST IN THE DIAGNOSIS OF TOXOPLASMOSIS. HAKIM RESEARCH JOURNAL[Internet]. 2002;5(3):219-224. Available from: https://sid.ir/paper/29175/en

IEEE: Copy

M. GHANE, M. ASMAR, ASHRAF ALSADAT NOUHI, A.R. ESMAEILI RASTAGHI, and GH.H. SEYDIPOOR, “ULTRAMICROSCOPIC STUDY OF TOXOPLASMA GONDII AND MECHANISM OF DYE TEST IN THE DIAGNOSIS OF TOXOPLASMOSIS,” HAKIM RESEARCH JOURNAL, vol. 5, no. 3, pp. 219–224, 2002, [Online]. Available: https://sid.ir/paper/29175/en

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