DETECTION AND PREVALENCE OF POLYOMA VIRUS BK AMONG IRANIAN KIDNEY TRANSPLANT PATIENTS BY A NOVEL NESTED-PCR

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SHENAGARI M.; RAVANSHAD M.; HOSSEINI S.Y.; GHANBARI R.

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Journal Paper

Paper Information

Journal: Iranian Red Crescent Medical Journal Year:2010 | Volume:12 | Issue:6 Page(s): 631-635

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Title

DETECTION AND PREVALENCE OF POLYOMA VIRUS BK AMONG IRANIAN KIDNEY TRANSPLANT PATIENTS BY A NOVEL NESTED-PCR

Author(s)

SHENAGARI M. | RAVANSHAD M. | HOSSEINI S.Y. | GHANBARI R. | Issue Writer Certificate

Keywords

BK VIRUS

RENAL TRANSPLANT RECIPIENTS

PREVALENCE

IRAN

Abstract

nephropathy and emerged as a cause of allograft failure linked to immunosuppressive regimens in RENAL TRANSPLANT RECIPIENTS. This study develops a sensitive PCR method to detect the viremia and viruria as well as the incidence of BK VIRUS infection in RENAL TRANSPLANT RECIPIENTS.Methods: A nested PCR method was developed and a total of 45 paired serum and urine samples from renal transplant recipient patients were collected and tested with the developed assay.Results: The threshold of the developed detection assay was 10 copies/ml of BKV DNA in samples. Our results also indicated that about 40% of the urine and 26.7% of serum samples were positive for BKV in renal transplant patients in this study.Conclusion: This Nested-PCR method was found a specific, sensitive and simple procedure for detection of viruria and viremia of BK VIRUS in RENAL TRANSPLANT RECIPIENTS.

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APA: Copy

SHENAGARI, M., RAVANSHAD, M., HOSSEINI, S.Y., & GHANBARI, R.. (2010). DETECTION AND PREVALENCE OF POLYOMA VIRUS BK AMONG IRANIAN KIDNEY TRANSPLANT PATIENTS BY A NOVEL NESTED-PCR. IRANIAN RED CRESCENT MEDICAL JOURNAL (IRCMJ), 12(6), 631-635. SID. https://sid.ir/paper/292335/en

Vancouver: Copy

SHENAGARI M., RAVANSHAD M., HOSSEINI S.Y., GHANBARI R.. DETECTION AND PREVALENCE OF POLYOMA VIRUS BK AMONG IRANIAN KIDNEY TRANSPLANT PATIENTS BY A NOVEL NESTED-PCR. IRANIAN RED CRESCENT MEDICAL JOURNAL (IRCMJ)[Internet]. 2010;12(6):631-635. Available from: https://sid.ir/paper/292335/en

IEEE: Copy

M. SHENAGARI, M. RAVANSHAD, S.Y. HOSSEINI, and R. GHANBARI, “DETECTION AND PREVALENCE OF POLYOMA VIRUS BK AMONG IRANIAN KIDNEY TRANSPLANT PATIENTS BY A NOVEL NESTED-PCR,” IRANIAN RED CRESCENT MEDICAL JOURNAL (IRCMJ), vol. 12, no. 6, pp. 631–635, 2010, [Online]. Available: https://sid.ir/paper/292335/en

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