DIFFERENTIATION MICRORNAS AFFECT STEMNESS STATUS OF USSCS

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JAMSHIDI ADEGANI F.; LANGROUDI L.; AREFIAN E.; SOLEIMANI MASOUD

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Journal Paper

Paper Information

Journal: Iranian Red Crescent Medical Journal Year:2011 | Volume:13 | Issue:10 Page(s): 726-734

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Information Journal Paper

Title

DIFFERENTIATION MICRORNAS AFFECT STEMNESS STATUS OF USSCS

Author(s)

JAMSHIDI ADEGANI F. | LANGROUDI L. | AREFIAN E. | SOLEIMANI MASOUD | Issue Writer Certificate

Keywords

MIRNA

MIR-145

LET7G

UNRESTRICTED SOMATIC STEM CELLS

EMBRYONIC STEM CELL

PLURIPOTENCY

LOCKED NUCLEIC ACID

Abstract

Background: MicroRNAs are endogenous non-coding RNAs with important regulatory and cell fate functions. Many studies have shown that several microRNAs are obviously up-regulated during stem cell differentiation. The question rises here is weather inhibiting differentiation will affect the stemness and self renewal status of stem cells.Methods: miRCURY ™LNA microRNA inhibitor (anti-miR-145 and anti-let7g) are a sequence-specific and chemically modified oligonucleotide that specifically target and knockdown MIR-145 and LET7G MIRNA molecules. UNRESTRICTED SOMATIC STEM CELLS (USSCs) were isolated from umbilical cord blood and treated with LNAs. The effect of anti-MIRNA transfection was assessed by quantitative real-time PCR.Results: Real-time PCR showed that LNA was efficiently introduced into the cells and reduced miR145 and LET7G expression levels to 40% and 10% in relation to corresponding scramble control, respectively. Gene expression analysis as to self renewal and expansion showed more than 3.5 fold up regulation in Oct4 in cells treated with mir145 inhibition. Similarly a significant up to 2.5 fold up-regulation in Oct4 and cMyc expression was observed in samples treated with anti-let7g.Conclusion: Suppression in differentiation inducing microRNAs (miR-145 and LET7G) can enhance the self renewal and stemness status of USSCs at transcriptional level.

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APA: Copy

JAMSHIDI ADEGANI, F., LANGROUDI, L., AREFIAN, E., & SOLEIMANI, MASOUD. (2011). DIFFERENTIATION MICRORNAS AFFECT STEMNESS STATUS OF USSCS. IRANIAN RED CRESCENT MEDICAL JOURNAL (IRCMJ), 13(10), 726-734. SID. https://sid.ir/paper/292477/en

Vancouver: Copy

JAMSHIDI ADEGANI F., LANGROUDI L., AREFIAN E., SOLEIMANI MASOUD. DIFFERENTIATION MICRORNAS AFFECT STEMNESS STATUS OF USSCS. IRANIAN RED CRESCENT MEDICAL JOURNAL (IRCMJ)[Internet]. 2011;13(10):726-734. Available from: https://sid.ir/paper/292477/en

IEEE: Copy

F. JAMSHIDI ADEGANI, L. LANGROUDI, E. AREFIAN, and MASOUD SOLEIMANI, “DIFFERENTIATION MICRORNAS AFFECT STEMNESS STATUS OF USSCS,” IRANIAN RED CRESCENT MEDICAL JOURNAL (IRCMJ), vol. 13, no. 10, pp. 726–734, 2011, [Online]. Available: https://sid.ir/paper/292477/en

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