CONSTRUCTION OF RECOMBINANT BACMID CONTAINING M2E-CTXB AND PRODUCING THE FUSION PROTEIN IN INSECT CELL LINES

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MIRZAEI NIMA; MOKHTARI AZAD TALAT; NATEGH RAKHSHANDEH; SOLEIMANJAHI HOORIEH; AMIRMOZAFARI NOUR

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Red Crescent Medical Journal Year:2014 | Volume:16 | Issue:2 Page(s): 1-7

Download Full-Text

View:

300

Download:

142

Cites:

Information Journal Paper

Title

CONSTRUCTION OF RECOMBINANT BACMID CONTAINING M2E-CTXB AND PRODUCING THE FUSION PROTEIN IN INSECT CELL LINES

Author(s)

Keywords

INFLUENZA VACCINES

BACULOVIRUS

CHOLERA TOXIN SUBUNIT B

Abstract

Background: Sequence variations in glycoproteins of influenza virus surface impel us to design new candidate vaccines yearly. Ectodomain of influenza M2 protein is a surface and highly conserved protein. M2e in INFLUENZA VACCINES may eliminate the need for changing vaccine formulation every year.Objectives: In this study, a recombinant BACULOVIRUS containing M2e and CHOLERA TOXIN SUBUNIT B fusion gene was generated with transposition process to express in large amounts in insect cell lines.Materials and Methods: M2e-ctxB fusion gene was created and cloned into pFastBac HT. The recombinant vector was transformed into DH10Bac cells to introduce the fusion gene into the bacmid DNA via a site-specific transposition process. The recombinant bacmid was then extracted from white colonies and further analyzed using PCR, DNA sequence analyzing, and indirect immunofluorescence assay.Results: PCR and DNA sequence analyzing results showed that the fusion gene was constructed as a single open reading frame and was successfully inserted into bacmid DNA. Moreover, indirect immunofluorescence results showed that the fusion gene was successfully expressed.Conclusions: BACULOVIRUS expression vector system is valuable to produce M2e based INFLUENZA VACCINES due to its simple utilization and ease of target gene manipulation. The expressed protein in such systems can improve the evaluating process of new vaccination strategies.

Cites

No record.

References

No record.

Cite

APA: Copy

MIRZAEI, NIMA, MOKHTARI AZAD, TALAT, NATEGH, RAKHSHANDEH, SOLEIMANJAHI, HOORIEH, & AMIRMOZAFARI, NOUR. (2014). CONSTRUCTION OF RECOMBINANT BACMID CONTAINING M2E-CTXB AND PRODUCING THE FUSION PROTEIN IN INSECT CELL LINES. IRANIAN RED CRESCENT MEDICAL JOURNAL (IRCMJ), 16(2), 1-7. SID. https://sid.ir/paper/292541/en

Vancouver: Copy

MIRZAEI NIMA, MOKHTARI AZAD TALAT, NATEGH RAKHSHANDEH, SOLEIMANJAHI HOORIEH, AMIRMOZAFARI NOUR. CONSTRUCTION OF RECOMBINANT BACMID CONTAINING M2E-CTXB AND PRODUCING THE FUSION PROTEIN IN INSECT CELL LINES. IRANIAN RED CRESCENT MEDICAL JOURNAL (IRCMJ)[Internet]. 2014;16(2):1-7. Available from: https://sid.ir/paper/292541/en

IEEE: Copy

NIMA MIRZAEI, TALAT MOKHTARI AZAD, RAKHSHANDEH NATEGH, HOORIEH SOLEIMANJAHI, and NOUR AMIRMOZAFARI, “CONSTRUCTION OF RECOMBINANT BACMID CONTAINING M2E-CTXB AND PRODUCING THE FUSION PROTEIN IN INSECT CELL LINES,” IRANIAN RED CRESCENT MEDICAL JOURNAL (IRCMJ), vol. 16, no. 2, pp. 1–7, 2014, [Online]. Available: https://sid.ir/paper/292541/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops