EVALUATION OF THE PROTECTIVE EFFECT OF SILIBININ AGAINST DIAZINON INDUCED HEPATOTOXICITY AND FREE-RADICAL DAMAGE IN RAT LIVER

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BEYDILLI HALIL; YILMAZ NIGAR; CETIN ESIN SAKALLI; TOPAL YASAR; CELIK OZGUR ILHAN; SAHIN CEM; TOPAL HATICE; CIGERCI IBRAHIM HAKKI; SOZEN HAMDI

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Journal: Iranian Red Crescent Medical Journal Year:2015 | Volume:17 | Issue:4 Page(s): 0-0

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Title

EVALUATION OF THE PROTECTIVE EFFECT OF SILIBININ AGAINST DIAZINON INDUCED HEPATOTOXICITY AND FREE-RADICAL DAMAGE IN RAT LIVER

Author(s)

Keywords

DIAZINON

OXIDATIVE STRESS

ANTIOXIDANTS

HISTOPATHOLOGY

LIVER

Abstract

Background: DIAZINON (0, 0-Diethyl 0- (1-6-methyl-2-isoprophyl 4 pyrimidinyl) phosphorothioate) (DI) is a very effective organophosphate pesticide, used widely in agriculture. Consequently, data on poisoning cases secondary to DI exposure are important. The DI may affect a variety of tissues, including LIVER. Silibinin is a pharmacologically active constitute of Silybum marianum, with documented antioxidant activity.Objectives: The aim of our study was to evaluate both histopathologically and biochemically whether silibinin is protective in DI induced LIVER damage.Materials and Methods: Thirty two Wistar albino rats were divided into four groups, as follows: 1) control group - oral corn oil was given; 2) DI group - rats were administered orally 335 mg/kg in the corn oil solution; 3) Silibinin group - 100 mg/kg/day silibinin was given alone orally, every 24 hours for 7 days; 4) Silibinin+DI group - DI plus silibinin was given. All rats were sacrificed at the end of experiment. Superoxide dismutases (SOD), glutathione peroxidase (GPX), nitric oxide (NO) and myeloperoxidase (MPO) were investigated in serum and LIVER tissue. In addition, serum aspartate aminotransferase (AST) and alanine aminotransferase (ALT) enzyme activities were evaluated. The LIVER tissue was evaluated histopathologically with Hematoxilin & Eosin dye.Results: Biochemically, ALT, AST, NO, MPO in serum and NO, MPO in LIVER tissue were found to be significantly higher in DI group, compared to control group (P<0.001). In Group Silibinin+DI, serum AST, ALT, NO, MPO levels were significantly lower (P<0.01), and both serum and tissue SOD activities were significantly higher, compared to DI group (P<0.001). DIAZINON induced histopathological changes in LIVER tissue were: severe sinusoidal dilatation, moderate disruption of the radial alignment of hepatocytes around the central vein, severe vacuolization in the hepatocyte cytoplasm, inflammation around central vein and portal region. In rats receiving both DI and silibinin, the DI induced changes accounted for less sinusoidal dilatation, vacuolization in the hepatocyte cytoplasm and the inflammation around central vein and portal region (P<0.05).Conclusions: The DI was found to induce LIVER damage by OXIDATIVE STRESS mechanisms. Silibinin reduced the OXIDATIVE STRESS by inducing antioxidant mechanisms, thereby showing protective effect against DI induced LIVER damage. Further studies with silibinin should be performed regarding DI toxicity.

Cites

THE EFFECT OF SILYMARIN SUPPLEMENTATION ON THE SERUM LEVELS OF INTERLEUKIN-6 AND C-REACTIVE PROTEIN FOLLOWING A SINGLE BOUT OF AEROBIC EXERCISE IN HEALTHY MEN

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APA: Copy

BEYDILLI, HALIL, YILMAZ, NIGAR, CETIN, ESIN SAKALLI, TOPAL, YASAR, CELIK, OZGUR ILHAN, SAHIN, CEM, TOPAL, HATICE, CIGERCI, IBRAHIM HAKKI, & SOZEN, HAMDI. (2015). EVALUATION OF THE PROTECTIVE EFFECT OF SILIBININ AGAINST DIAZINON INDUCED HEPATOTOXICITY AND FREE-RADICAL DAMAGE IN RAT LIVER. IRANIAN RED CRESCENT MEDICAL JOURNAL (IRCMJ), 17(4), 0-0. SID. https://sid.ir/paper/292961/en

Vancouver: Copy

BEYDILLI HALIL, YILMAZ NIGAR, CETIN ESIN SAKALLI, TOPAL YASAR, CELIK OZGUR ILHAN, SAHIN CEM, TOPAL HATICE, CIGERCI IBRAHIM HAKKI, SOZEN HAMDI. EVALUATION OF THE PROTECTIVE EFFECT OF SILIBININ AGAINST DIAZINON INDUCED HEPATOTOXICITY AND FREE-RADICAL DAMAGE IN RAT LIVER. IRANIAN RED CRESCENT MEDICAL JOURNAL (IRCMJ)[Internet]. 2015;17(4):0-0. Available from: https://sid.ir/paper/292961/en

IEEE: Copy

HALIL BEYDILLI, NIGAR YILMAZ, ESIN SAKALLI CETIN, YASAR TOPAL, OZGUR ILHAN CELIK, CEM SAHIN, HATICE TOPAL, IBRAHIM HAKKI CIGERCI, and HAMDI SOZEN, “EVALUATION OF THE PROTECTIVE EFFECT OF SILIBININ AGAINST DIAZINON INDUCED HEPATOTOXICITY AND FREE-RADICAL DAMAGE IN RAT LIVER,” IRANIAN RED CRESCENT MEDICAL JOURNAL (IRCMJ), vol. 17, no. 4, pp. 0–0, 2015, [Online]. Available: https://sid.ir/paper/292961/en

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