IN VITRO CULTIVATION, CHARACTERIZATION AND OSTEOGENIC DIFFERENTIATION OF STEM CELLS FROM HUMAN EXFOLIATED DECIDUOUS TEETH ON 3D PRINTED POLYLACTIC ACID SCAFFOLDS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

ISLAM AYLIN; MAMMADOV EMIL; KENDIRCI REMZIYE; AYTAC ERSIN; CETINER SERAP; VATANSEVER HAFIZE SEDA

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Red Crescent Medical Journal Year:2017 | Volume:19 | Issue:8 Page(s): 0-0

Download Full-Text

View:

Download:

Cites:

Information Journal Paper

Title

IN VITRO CULTIVATION, CHARACTERIZATION AND OSTEOGENIC DIFFERENTIATION OF STEM CELLS FROM HUMAN EXFOLIATED DECIDUOUS TEETH ON 3D PRINTED POLYLACTIC ACID SCAFFOLDS

Author(s)

Keywords

CELL DIFFERENTIATION

DENTISTRY

STEM CELL

TISSUE ENGINEERING

Abstract

Background: TISSUE ENGINEERING mainly focuses on creating appropriate conditions for the regeneration of tissues. Scaffolds, signal molecules, and STEM CELLs interact with each other and compose the essential components of this field.Objectives: This study aimed at investigating the osteogenic induction ability of PLA Poly Lactic Acid (PLA) scaffolds and comparing the osteogenic differentiation behavior of STEM CELLs from Human Exfoliated Deciduous Teeth (hSHEDs) in standard culture medium and on PLA scaffolds.Methods: The current clinical experimental study was conducted between April 2016 and October 2016 at the Near East University cell culture laboratory located in North Cyprus. The pulp tissues of deciduous teeth (non-decayed and in the absence of abscess, fistula or periapical lesion) were sampled from 10 healthy children aged between 6 and 11 years. The isolated hSHEDs were divided to 4 groups. The control group/Group1 consisted of cells, which were cultivated in standard culture medium, and Group2 cells were differentiated into an osteogenic lineage using osteogenic differentiation medium. Group 3 represented the non-differentiated group, which was transferred onto three dimensional (3D) printed PLA scaffolds and Group 4 cells were differentiated to the osteogenic lineage and transferred onto 3D printed PLA scaffolds. All groups were analyzed immunohistochemically and by immune-labeling, and were evaluated semi-quantitatively using the HSCORE.Results: Cultivation of hSHEDS on PLA scaffolds was assessed for 14 and 21 days; osteogenic differentiation was detected both histochemically and immunohistochemically. Generally, Osteocalcin (OCN) immunoreactivities were higher than Osteonectin (ON) immunoreactions in all groups. Despite higher OCN immunoreactivities, the intensities of OCN between 14 days and 21 days in group 4 (497.3 ± 0.57% and 486.7 ± 5.77%, respectively) were similar (P>0.05). While the intensity of ON was 280.010% in group 4, in group 2 the intensity of ON was 206.7 ± 5.77%, and on the 14th day the results were statistically significant (P<0.0001).Conclusions: Poly lactic acid is a suitable scaffold material for osteogenic induction of the hSHEDs. The expression patterns of both markers showed that a 14-day cultivation period is adequate for hSHEDs with/without PLA scaffolds to differentiate into osteoblasts.

Cites

No record.

References

Cite

APA: Copy

ISLAM, AYLIN, MAMMADOV, EMIL, KENDIRCI, REMZIYE, AYTAC, ERSIN, CETINER, SERAP, & VATANSEVER, HAFIZE SEDA. (). . , (), -. SID. https://sid.ir/paper/293543/en

Vancouver: Copy

ISLAM AYLIN, MAMMADOV EMIL, KENDIRCI REMZIYE, AYTAC ERSIN, CETINER SERAP, VATANSEVER HAFIZE SEDA. . [Internet]. ;():-. Available from: https://sid.ir/paper/293543/en

IEEE: Copy

AYLIN ISLAM, EMIL MAMMADOV, REMZIYE KENDIRCI, ERSIN AYTAC, SERAP CETINER, and HAFIZE SEDA VATANSEVER, “,” , vol. , no. , pp. –, , [Online]. Available: https://sid.ir/paper/293543/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops