مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Title

EVALUATION OF THE EFFECT OF PROBIOTICS ISOLATED FROM TRADITIONAL DAIRYPRODUCTS ON THE EXPRESSION OF STX1 AND STX2 GENES OF VEROTOXIGENICESCHERICHIA COLI (VTEC) IN LABORATORY CONDITIONS

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Abstract

 Background: Lactobacilli are commonly used organisms in the production of PROBIOTICs. Objectives: The present study was conducted to examine the effect of PROBIOTICs isolated from dairy products on the EXPRESSION ofStx1 and STX2 GENEs in VEROTOXIGENIC E. coli (VTEC). Methods: This experimental study was conducted during April and January 2016 in East Azerbaijan, Iran. At first, Hundred samplesof traditional dairy products were collected. Then, using standard phenotypic and genotypic methods, the Lactobacillus casei (L. casei) and lactobacillus (L. plantarum) were studied. Subsequently, these strains were examined along with the pathogenic bacteriastrain of ESCHERICHIA COLI (E. coli) (O157: H7) by neighboring cultivation. After extraction of mRNA, the EXPRESSION of these GENEs wasdetermined using real-time PCR. Results: The results revealed that L. casei reduces the EXPRESSION of SHIGA TOXIN (Stx1 and STX2) GENEs more compared to L. plantarum(P = 0. 001). Moreover, it was found that both Lactobacilli reduce the EXPRESSION of STX1 GENEs more than STX2. Conclusions: PROBIOTICs could be used to prevent and control intestinal diseases caused by E. coli.

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    APA: Copy

    AHMADIZADEH, CHANGIZ, & MIRZAEI, HAMID. (2018). EVALUATION OF THE EFFECT OF PROBIOTICS ISOLATED FROM TRADITIONAL DAIRYPRODUCTS ON THE EXPRESSION OF STX1 AND STX2 GENES OF VEROTOXIGENICESCHERICHIA COLI (VTEC) IN LABORATORY CONDITIONS. IRANIAN RED CRESCENT MEDICAL JOURNAL (IRCMJ), 20(3), 0-0. SID. https://sid.ir/paper/293704/en

    Vancouver: Copy

    AHMADIZADEH CHANGIZ, MIRZAEI HAMID. EVALUATION OF THE EFFECT OF PROBIOTICS ISOLATED FROM TRADITIONAL DAIRYPRODUCTS ON THE EXPRESSION OF STX1 AND STX2 GENES OF VEROTOXIGENICESCHERICHIA COLI (VTEC) IN LABORATORY CONDITIONS. IRANIAN RED CRESCENT MEDICAL JOURNAL (IRCMJ)[Internet]. 2018;20(3):0-0. Available from: https://sid.ir/paper/293704/en

    IEEE: Copy

    CHANGIZ AHMADIZADEH, and HAMID MIRZAEI, “EVALUATION OF THE EFFECT OF PROBIOTICS ISOLATED FROM TRADITIONAL DAIRYPRODUCTS ON THE EXPRESSION OF STX1 AND STX2 GENES OF VEROTOXIGENICESCHERICHIA COLI (VTEC) IN LABORATORY CONDITIONS,” IRANIAN RED CRESCENT MEDICAL JOURNAL (IRCMJ), vol. 20, no. 3, pp. 0–0, 2018, [Online]. Available: https://sid.ir/paper/293704/en

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