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Information Journal Paper

Title

DETERMINATION OF DIFFERENT FLUOROQUINOLONE MECHANISMS AMONGCLINICAL ISOLATES OF ACINETOBACTER BAUMANNII IN TEHRAN, IRAN (BRIEF REPORT)

Pages

  0-0

Abstract

 Background: Acinetobacter baumannii isolates resistant to FLUOROQUINOLONES, such as levofloxacin and ciprofloxacin are being increasinglydeveloped every day. Objectives: In this study, ciprofloxacin resistance in A. baumannii isolates was determined by the presence or absence of effluxpumpinhibitors, as the efflux pumps play an important role in the creation of ciprofloxacin resistance. Methods: One hundred and three Acinetobacter isolates were collected from ventilator-associated pneumonia (VAP) and burn patientsof Tehran hospitals, Iran, during six months of 2014. Susceptibility rates of the isolates to levofloxacin and ciprofloxacin antibioticswere assessed using the agar disk diffusion and broth microdilution. The effects of the efflux pump inhibitors includingphenylalanine-arginine -naphthylamide (PA N) and 1-(1-naphtylmethyl)-piperazine (NMP) on ciprofloxacin resistance were investigated. Further, the quinolone resistance qnrA, qnrB, qnrS, and adeABC genes were evaluated using the polymerase chain reaction(PCR) test. Finally, to examine the mutation in quinolone resistance-determining regions, the PCR products of the gyrA and parCgenes were sequenced. Results: According to the results of the antibiogram test, 74. 7% and 33% of the studied isolates were resistant to ciprofloxacin andlevofloxacin, respectively. Also, there was a significant relationship between the type of the specimen and resistance to ciprofloxacin(P = 0. 02) and resistance to levofloxacin (P = 0. 04). As for the synergistic study of the inhibitors with ciprofloxacin, the reduction ofminimum inhibitory concentration (MIC) was observed in 40% and 56. 6% of the isolates in the presence of PA N and NMP, respectively. The prevalence rates of qnrA, qnrB, qnrS, AdeA, AdeB, and AdeC genes were 0%, 0%, 3. 9%, 100%, 100% and 100%, respectively. In allthe resistant isolates, mutation of in the gyrA gene was observed, but no mutation was seen in the parC gene. Conclusions: The presence of the efflux pumps and the gyrA gene mutation are still considered as the most important factors causingfluoroquinolone resistance; however, identification of the qnr genes for the first time in Tehran hospitals, Iran, can lead to furtherconcerns in the future.

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    APA: Copy

    KHAYAT, HATEF, SADEGHIFARD, NOURKHODA, PAKZAD, IRAJ, Azimi, Lila, DELFANI, SOMAYEH, SAYEHMIRI, KOROUSH, SOROUSH, SETAREH, BOGDANOVIC, LIDIJA, & TAHERIKALANI, MOROVAT. (2017). DETERMINATION OF DIFFERENT FLUOROQUINOLONE MECHANISMS AMONGCLINICAL ISOLATES OF ACINETOBACTER BAUMANNII IN TEHRAN, IRAN (BRIEF REPORT). IRANIAN RED CRESCENT MEDICAL JOURNAL (IRCMJ), 19(9), 0-0. SID. https://sid.ir/paper/293737/en

    Vancouver: Copy

    KHAYAT HATEF, SADEGHIFARD NOURKHODA, PAKZAD IRAJ, Azimi Lila, DELFANI SOMAYEH, SAYEHMIRI KOROUSH, SOROUSH SETAREH, BOGDANOVIC LIDIJA, TAHERIKALANI MOROVAT. DETERMINATION OF DIFFERENT FLUOROQUINOLONE MECHANISMS AMONGCLINICAL ISOLATES OF ACINETOBACTER BAUMANNII IN TEHRAN, IRAN (BRIEF REPORT). IRANIAN RED CRESCENT MEDICAL JOURNAL (IRCMJ)[Internet]. 2017;19(9):0-0. Available from: https://sid.ir/paper/293737/en

    IEEE: Copy

    HATEF KHAYAT, NOURKHODA SADEGHIFARD, IRAJ PAKZAD, Lila Azimi, SOMAYEH DELFANI, KOROUSH SAYEHMIRI, SETAREH SOROUSH, LIDIJA BOGDANOVIC, and MOROVAT TAHERIKALANI, “DETERMINATION OF DIFFERENT FLUOROQUINOLONE MECHANISMS AMONGCLINICAL ISOLATES OF ACINETOBACTER BAUMANNII IN TEHRAN, IRAN (BRIEF REPORT),” IRANIAN RED CRESCENT MEDICAL JOURNAL (IRCMJ), vol. 19, no. 9, pp. 0–0, 2017, [Online]. Available: https://sid.ir/paper/293737/en

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