PREPARATION OF ENRICHED IMMUNOGLOBULIN M AND IMMUNOGLOBULIN A FROM HUMAN PLASMA

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MOUSAVI HOSSEINI K.; POURMOKHTAR M.; DINARVAND R.; REZVAN H.; REZVAN H.; JALILI M.A.

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Journal Paper

Paper Information

Journal: Medical Journal of The Islamic Republic of Iran Year:2004 | Volume:17 | Issue:4 Page(s): 315-318

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Title

PREPARATION OF ENRICHED IMMUNOGLOBULIN M AND IMMUNOGLOBULIN A FROM HUMAN PLASMA

Author(s)

Keywords

IMMUNOGLOBULIN

IGM

IGA; ENRICHMENT

ION EXCHANGE CHROMATOGRAPHY

Abstract

As IGM and IgA-enriched preparations are needed to complete the immunotherapeutic spectrum, a simple procedure is described for the preparation of IGM and IgA enriched IMMUNOGLOBULINs. Fraction ill which was prepared by cold ethanol fractionation was treated by octanoic acid followed by ethanol precipitation and ion-exchange chromatography using Sephadex DEAE A-50 and 0.1 M tris-0.35M NaCl buffer, pH 8.1, resulting in recovery of 85% IGM, 84% IgA and 33% IgG. The comparison of our results with IMMUNOGLOBULINs" percentage in plasma indicates that IGM and IgA-enrichment was obtained by three times.

Cites

PASTEURIZATION OF IGM-ENRICHED IMMUNOGLOBULIN

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APA: Copy

MOUSAVI HOSSEINI, K., POURMOKHTAR, M., DINARVAND, R., REZVAN, H., REZVAN, H., & JALILI, M.A.. (2004). PREPARATION OF ENRICHED IMMUNOGLOBULIN M AND IMMUNOGLOBULIN A FROM HUMAN PLASMA. MEDICAL JOURNAL OF THE ISLAMIC REPUBLIC OF IRAN (MJIRI), 17(4), 315-318. SID. https://sid.ir/paper/293981/en

Vancouver: Copy

MOUSAVI HOSSEINI K., POURMOKHTAR M., DINARVAND R., REZVAN H., REZVAN H., JALILI M.A.. PREPARATION OF ENRICHED IMMUNOGLOBULIN M AND IMMUNOGLOBULIN A FROM HUMAN PLASMA. MEDICAL JOURNAL OF THE ISLAMIC REPUBLIC OF IRAN (MJIRI)[Internet]. 2004;17(4):315-318. Available from: https://sid.ir/paper/293981/en

IEEE: Copy

K. MOUSAVI HOSSEINI, M. POURMOKHTAR, R. DINARVAND, H. REZVAN, H. REZVAN, and M.A. JALILI, “PREPARATION OF ENRICHED IMMUNOGLOBULIN M AND IMMUNOGLOBULIN A FROM HUMAN PLASMA,” MEDICAL JOURNAL OF THE ISLAMIC REPUBLIC OF IRAN (MJIRI), vol. 17, no. 4, pp. 315–318, 2004, [Online]. Available: https://sid.ir/paper/293981/en

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