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Information Journal Paper

Title

CYTOGENETIC AND MOLECULAR GENETIC ANALYSIS OF DICENTRIC Y CHROMOSOME AND ITS RELATION TO MALE AZOOSPERMIA

Pages

  57-64

Abstract

 Background: Cytogenetic analysis, Y-chromosome microdeletion screening, FISH techniques and other genetic methods have helped in finding the causes of infertility in azoospermic or severe oligoazoospermic cases in the last decade.Objective: In the present study, we characterized an abnormal Y-chromosome, detected as a mosaic in an azoospermic male ascertained for infertility.Materials and Methods: Chromosome analysis, using G, Q and C banding techniques and FISH analyses with several different DNA probes specific for Y and X chromosome sequences [XY centromeric α-satellite, Y non-a-satellite III, LSI-probes of the Y chromosome, WCP of Chromosome Y, SRY gene, subtelomeric Xp and Yp, which cover the SHOX (short stature-homeobox containing) gene, and subtelomeric Xq and Yq probes] were performed. A total of 20 sequence tagged sites were analyzed using primer sets specific for the Y-chromosome microdeletion loci. The primers were chosen to cover AZFa, AZFb, and AZFc regions as well as the SRY gene.Results: Chromosome analysis revealed a gonosomal MOSAICISM of monosomy X (51%) and a pseudodicentric Y (49%) chromosome: mos 45, X/46,X psu dic (Y) (qter®p11.32 :: p11.2®qter). Molecular genetic studies did not show deletions in the AZFabc regions, but a deletion was found in the short arms of the DICENTRIC Y CHROMOSOME. One of the SRY genes was also missing.Conclusion: The AZOOSPERMIA in this patient could be explained by either the presence of an abnormal Y-chromosome that cannot form a sex vesicle (which appears to be necessary for the completion of meiosis process and the formation of sperm), or the presence of the 45, X cell line.

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    • Cite

    APA: Copy

    OMRANI, M.D., KARIMZAD HAGH, J., KLEIN, W., & GEBAUER, J.. (2008). CYTOGENETIC AND MOLECULAR GENETIC ANALYSIS OF DICENTRIC Y CHROMOSOME AND ITS RELATION TO MALE AZOOSPERMIA. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), 6(2), 57-64. SID. https://sid.ir/paper/295152/en

    Vancouver: Copy

    OMRANI M.D., KARIMZAD HAGH J., KLEIN W., GEBAUER J.. CYTOGENETIC AND MOLECULAR GENETIC ANALYSIS OF DICENTRIC Y CHROMOSOME AND ITS RELATION TO MALE AZOOSPERMIA. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE)[Internet]. 2008;6(2):57-64. Available from: https://sid.ir/paper/295152/en

    IEEE: Copy

    M.D. OMRANI, J. KARIMZAD HAGH, W. KLEIN, and J. GEBAUER, “CYTOGENETIC AND MOLECULAR GENETIC ANALYSIS OF DICENTRIC Y CHROMOSOME AND ITS RELATION TO MALE AZOOSPERMIA,” INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), vol. 6, no. 2, pp. 57–64, 2008, [Online]. Available: https://sid.ir/paper/295152/en

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