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Information Journal Paper

Title

EFFECT OF VARIOUS CONCENTRATIONS OF MINIMAL ESSENTIAL MEDIUM VITAMINS (MEM VITAMINS) ON DEVELOPMENT OF SHEEP OOCYTES DURING IN-VITRO MATURATION

Pages

  93-98

Abstract

 Background: Improvements in culture media formulations have led to an increase in the ability of SHEEP embryo in culture throughout the preimplantation period.Objective: This study was carried out to evaluate the effects of various concentrations of MEM VITAMINS during in vitro maturation of SHEEP oocytes and subsequent EMBRYO DEVELOPMENT.Materials and Methods: SHEEP ovaries were collected from a slaughterhouse and transported to the laboratory. Oocytes were matured in SOF medium supplemented with, eCG, hCG and EGF in various concentrations of MEM VITAMINS (control, 0.5, 1 and 1.5 ´) for 24h. The cumulus oocyte compelex (COCs) were co-incubated with epididymal spermatozoa of post mortem rams in synthetic oviduct fluid fertilization (SOFF) medium with 10% heat inactivated estrous SHEEP serum for 18h. Embryos were cultured in synthetic oviduct fluid culture 1 (SOFC1) medium for 48h followed by cultured in synthetic oviduct fluid culture 2 (SOFC2) medium for six days.Results: Addition of 0.5 and 1 ´ MEM VITAMINS significantly increased (P<0.05) overall blastocyst development (21.62% and 22.33%; respectively) compared with 1.5 ´ MEM VITAMINS (15.59%), but there was no difference between control, 0.5 and 1´MEM vitamins in the percentage of embryos successfully developing to the blastocyst stage (19.50%, 21.62% and 22.33% respectively).Conclusion: It seems that addition of 1.5 ´ of MEM VITAMINS has detrimental effect on blastocyst rate.

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    APA: Copy

    KAFILZADEH, FAROKH, KARAMI SHABANKAREH, HAMED, & SOLTANI, LEILA. (2012). EFFECT OF VARIOUS CONCENTRATIONS OF MINIMAL ESSENTIAL MEDIUM VITAMINS (MEM VITAMINS) ON DEVELOPMENT OF SHEEP OOCYTES DURING IN-VITRO MATURATION. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), 10(2), 93-98. SID. https://sid.ir/paper/295868/en

    Vancouver: Copy

    KAFILZADEH FAROKH, KARAMI SHABANKAREH HAMED, SOLTANI LEILA. EFFECT OF VARIOUS CONCENTRATIONS OF MINIMAL ESSENTIAL MEDIUM VITAMINS (MEM VITAMINS) ON DEVELOPMENT OF SHEEP OOCYTES DURING IN-VITRO MATURATION. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE)[Internet]. 2012;10(2):93-98. Available from: https://sid.ir/paper/295868/en

    IEEE: Copy

    FAROKH KAFILZADEH, HAMED KARAMI SHABANKAREH, and LEILA SOLTANI, “EFFECT OF VARIOUS CONCENTRATIONS OF MINIMAL ESSENTIAL MEDIUM VITAMINS (MEM VITAMINS) ON DEVELOPMENT OF SHEEP OOCYTES DURING IN-VITRO MATURATION,” INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), vol. 10, no. 2, pp. 93–98, 2012, [Online]. Available: https://sid.ir/paper/295868/en

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