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Title

HYDROSTATIC PRESSURE IMPROVES IN-VITRO MATURATION OF OOCYTES DERIVED FROM VITRIFIED-WARMED MOUSE OVARIES

Pages

  257-264

Abstract

 Background: Cryopreservation has limited successes and IN-VITRO MATURATION is used to improve its results. HYDROSTATIC PRESSURE (HP) plays an important role in follicular development. Objective: This study was designed to examine the effects of HP on IN-VITRO MATURATION of oocytes and CELL DEATH in cumulus cells derived from vitrified-warmed MOUSE ovaries.Materials and Methods: Preovulatory follicles were harvested from non-vitrified and vitrified-warmed 6-8 week-old female NMRI MOUSE ovaries and randomly assigned to following groups: non-vitrified (control), non-vitrified with HP exposure (treatment I), vitrified-warmed (treatment II) and vitrified-warmed with HP exposure (treatment III). The follicles of treatments I and III were subjected to HP (20 mmHg) for 30 min and after that all groups were cultured for 24h and assessed for IN-VITRO MATURATION of oocytes. The viability and apoptosis of cumulus cells and oocytes were assessed using supravital nuclear staining and TUNEL assay, respectively.Results: Oocytes harvested follicles in both control and treatment II had a significantly lower percentage of metaphase II oocytes (MII) than the treatment I and III (23.5±3.1, 15.03±4.6 and 32.7±3.2, 25.5±4.6; respectively) (p<0.05). Viability of the cumulus cells reduced in treatment I, II and III (83.4, 83.3 and 77.7%) compared to control (86.9%), (p<0.05). The apoptotic index in cumulus and oocyte complexes in treatments I and III (10.7±0.8 and 15.3±0.8) was higher than in control and treatment II (6.7±0.5 and 9.7±0.5) (p<0.05).Conclusion: These results demonstrate that HP had a mild effect on CELL DEATH incidence in cumulus cells without any effect on oocyte. However, it can be used as a mechanical force to improve IN-VITRO MATURATION of oocytes derived from vitrified-warmed MOUSE ovaries.

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    APA: Copy

    RASHIDI, ZAHRA, AZADBAKHT, MEHRI, & KHAZAEI, MOZAFAR. (2012). HYDROSTATIC PRESSURE IMPROVES IN-VITRO MATURATION OF OOCYTES DERIVED FROM VITRIFIED-WARMED MOUSE OVARIES. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), 10(3), 257-264. SID. https://sid.ir/paper/295883/en

    Vancouver: Copy

    RASHIDI ZAHRA, AZADBAKHT MEHRI, KHAZAEI MOZAFAR. HYDROSTATIC PRESSURE IMPROVES IN-VITRO MATURATION OF OOCYTES DERIVED FROM VITRIFIED-WARMED MOUSE OVARIES. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE)[Internet]. 2012;10(3):257-264. Available from: https://sid.ir/paper/295883/en

    IEEE: Copy

    ZAHRA RASHIDI, MEHRI AZADBAKHT, and MOZAFAR KHAZAEI, “HYDROSTATIC PRESSURE IMPROVES IN-VITRO MATURATION OF OOCYTES DERIVED FROM VITRIFIED-WARMED MOUSE OVARIES,” INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), vol. 10, no. 3, pp. 257–264, 2012, [Online]. Available: https://sid.ir/paper/295883/en

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