EFFECT OF COMBINED DENSITY GRADIENT CENTRIFUGATION ON X- AND Y- SPERM SEPARATION AND CHROMATIN INTEGRITY

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ESMAEILPOUR TAHEREH; ELYASI LEILA; BAHMANPOUR SOGHRA; GHANNADI ALIREZA; MONABATI AHMAD; DEHGHANI FARZANEH; KAZEROONI MARJANEH

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Journal: International Journal of Reproductive BioMedicine Year:2012 | Volume:10 | Issue:5 Page(s): 435-440

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Title

EFFECT OF COMBINED DENSITY GRADIENT CENTRIFUGATION ON X- AND Y- SPERM SEPARATION AND CHROMATIN INTEGRITY

Author(s)

Keywords

ALBUMIN GRADIENT

DOUBLE-LABELED FLUORESCENCE IN SITU HYBRIDIZATION

SPERM SEPARATION

SEX RATIO

SPERM CHROMATIN

Abstract

Background: It has been claimed that by using different washing methods, the sperms can be separated according to size, motility, density, chromosomal content and surface markings and charge. These methods also reduce SPERM CHROMATIN deficiencies and screen the sperms before applying in assisted reproduction techniques.Objective: This study compared simple density gradient methods and a combined method with albumin density gradient and PureSperm separation (alb/PureSperm) for sex preselection by double fluorescence in situ hybridization (FISH) versus chromomycin A3 staining to determine chromatin integrity.Materials and Methods: 30 normal semen samples were prepared with PureSperm, ALBUMIN GRADIENTs and alb/PureSperm. All samples were then stained by FISH and chromomycin A3. The results were compared with SPSS 11.5 and the Kruskal-Wallis test.Results: The proportion of X-bearing spermatozoa by PureSperm separation (47.58±5.67) and Y-bearing spermatozoa by ALBUMIN GRADIENT (46.13±3.83) methods were slightly higher than in putative normal sperm samples (1: 1), but there were no significant differences in the X- or Y- bearing spermatozoa counts among the three methods. ALBUMIN GRADIENT separation tended to underestimate abnormal spermatozoa compared to PureSperm and combined alb/PureSperm.Conclusion: Routine separation methods slightly enriched X- or Y- bearing spermatozoa, but the differences were not significant for clinical purposes. The combined alb/PureSperm method had no advantages for assessing SEX RATIO or chromatin integrity compared to simpler gradient methods.

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APA: Copy

ESMAEILPOUR, TAHEREH, ELYASI, LEILA, BAHMANPOUR, SOGHRA, GHANNADI, ALIREZA, MONABATI, AHMAD, DEHGHANI, FARZANEH, & KAZEROONI, MARJANEH. (2012). EFFECT OF COMBINED DENSITY GRADIENT CENTRIFUGATION ON X- AND Y- SPERM SEPARATION AND CHROMATIN INTEGRITY. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), 10(5), 435-440. SID. https://sid.ir/paper/295921/en

Vancouver: Copy

ESMAEILPOUR TAHEREH, ELYASI LEILA, BAHMANPOUR SOGHRA, GHANNADI ALIREZA, MONABATI AHMAD, DEHGHANI FARZANEH, KAZEROONI MARJANEH. EFFECT OF COMBINED DENSITY GRADIENT CENTRIFUGATION ON X- AND Y- SPERM SEPARATION AND CHROMATIN INTEGRITY. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE)[Internet]. 2012;10(5):435-440. Available from: https://sid.ir/paper/295921/en

IEEE: Copy

TAHEREH ESMAEILPOUR, LEILA ELYASI, SOGHRA BAHMANPOUR, ALIREZA GHANNADI, AHMAD MONABATI, FARZANEH DEHGHANI, and MARJANEH KAZEROONI, “EFFECT OF COMBINED DENSITY GRADIENT CENTRIFUGATION ON X- AND Y- SPERM SEPARATION AND CHROMATIN INTEGRITY,” INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), vol. 10, no. 5, pp. 435–440, 2012, [Online]. Available: https://sid.ir/paper/295921/en

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