MULTIPLEX PCR BASED SCREENING FOR MICRODELETIONS IN AZOOSPERMIA FACTOR REGION OF Y CHROMOSOME IN AZOOSPERMIC AND SEVERE OLIGOZOOSPERMIC SOUTH INDIAN MEN

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SUGANTHI RAMASWAMY; VIJESH V.V.; JAYACHANDRAN SANJAY; FATHIMA BENAZIR JAHANGIR ALI

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Journal Paper

Paper Information

Journal: International Journal of Reproductive BioMedicine Year:2013 | Volume:11 | Issue:3 Page(s): 219-226

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Title

MULTIPLEX PCR BASED SCREENING FOR MICRODELETIONS IN AZOOSPERMIA FACTOR REGION OF Y CHROMOSOME IN AZOOSPERMIC AND SEVERE OLIGOZOOSPERMIC SOUTH INDIAN MEN

Author(s)

SUGANTHI RAMASWAMY | VIJESH V.V. | JAYACHANDRAN SANJAY | FATHIMA BENAZIR JAHANGIR ALI | Issue Writer Certificate

Keywords

Y CHROMOSOMAL MICRODELETIONS

AZF REGION

MALE INFERTILITY

MULTIPLEX PCR

Abstract

Background: Y chromosomal microdeletion is an important genetic disorder, which may arise due to intrachromosomal recombination between homologous sequences in the male specific region of the human Y chromosome. It is frequently associated with the quantitative reduction of sperm. The screening for Y CHROMOSOMAL MICRODELETIONS has a great clinical value. Objective: To develop a sequence tagged site (STS) based MULTIPLEX PCR protocol, which could be specific for the rapid detection of AZF deletions and thereby estimating the frequency of AZF sub deletions in infertile South Indian men. Materials and Methods: In the current study, PCR based Y chromosomal microdeletion screening analysis was performed in 75 men including 30 non-obstructive azoospermic men, 20 severe oligozoospermic, and 25 normozoospermic fertile men (controls) using 15 known STS primer pairs mapped within the AZF locus. Deletion frequency was estimated after successful PCR amplification. Results: We designed and optimized a STS based MULTIPLEX PCR protocol, which could be helpful for the clinicians to detect the Y chromosomal deletions rapidly and specifically. In our study, we estimated an overall deletion frequency of 36%. Among these 12 (40%) were azoospermic and 6 (30%) were oligozoospermic. No microdeletions were observed in normozoospermic fertile men. Conclusion: Our Study emphasizes the fact that Y chromosomal microdeletion screening tests are unavoidable in the workup of idiopathic MALE INFERTILITY. Mandatory screening for Y deletions should be done in all azoospermic and severe oligozoospermic patients before undergoing assisted reproductive technology.

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APA: Copy

SUGANTHI, RAMASWAMY, VIJESH, V.V., JAYACHANDRAN, SANJAY, & FATHIMA BENAZIR, JAHANGIR ALI. (2013). MULTIPLEX PCR BASED SCREENING FOR MICRODELETIONS IN AZOOSPERMIA FACTOR REGION OF Y CHROMOSOME IN AZOOSPERMIC AND SEVERE OLIGOZOOSPERMIC SOUTH INDIAN MEN. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), 11(3), 219-226. SID. https://sid.ir/paper/295960/en

Vancouver: Copy

SUGANTHI RAMASWAMY, VIJESH V.V., JAYACHANDRAN SANJAY, FATHIMA BENAZIR JAHANGIR ALI. MULTIPLEX PCR BASED SCREENING FOR MICRODELETIONS IN AZOOSPERMIA FACTOR REGION OF Y CHROMOSOME IN AZOOSPERMIC AND SEVERE OLIGOZOOSPERMIC SOUTH INDIAN MEN. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE)[Internet]. 2013;11(3):219-226. Available from: https://sid.ir/paper/295960/en

IEEE: Copy

RAMASWAMY SUGANTHI, V.V. VIJESH, SANJAY JAYACHANDRAN, and JAHANGIR ALI FATHIMA BENAZIR, “MULTIPLEX PCR BASED SCREENING FOR MICRODELETIONS IN AZOOSPERMIA FACTOR REGION OF Y CHROMOSOME IN AZOOSPERMIC AND SEVERE OLIGOZOOSPERMIC SOUTH INDIAN MEN,” INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), vol. 11, no. 3, pp. 219–226, 2013, [Online]. Available: https://sid.ir/paper/295960/en

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