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Information Journal Paper

Title

ZINC AND LOW-DOSE OF CADMIUM PROTECT SERTOLI CELLS AGAINST TOXIC-DOSE OF CADMIUM: THE ROLE OF METALLOTHIONEIN

Pages

  487-494

Abstract

 Background: The impact of CADMIUM (Cd) on male infertility may be related to the interaction with metal-binding proteins known as METALLOTHIONEINs (Mts). Trace elements like ZINC (Zn) have protective effects on testicular damage induced by Cd.Objective: We determined the effect of Zn and low-dose Cd pre-treatment on the expression of Mt1 and Mt2 genes on testicular SERTOLI CELLS.Materials and Methods: The cultured TM4 mouse SERTOLI CELLS were treated with 50 mM ZnSO4 (Zn pre-treated group; ZnPG), 2 μM CdCl2 (Cd pre-treated group; CdPG), or distilled water (DW pre-treated group; DWPG). After 18 hour, all of these groups were exposed to 100 mM CdCl2 for different periods of time (1, 2, 3, and 6 hours). There was also a control group for all three groups, which was treated only with distilled water (without Cd or Zn pre-treatment). Cellular viability, Zn and Cd concentrations and GENE EXPRESSION were assessed by MTT, atomic absorption spectrometry and real time PCR methods, respectively.Results: The expression of Mt1 and Mt2 genes in ZnPG, CdPG, and DWPG was greater than the control group (p=0.02 and p=0.01, respectively). Cd concentrations in CdPG and DWPG were greater than the control group (p=0.00). Expression of both genes in ZnPG and CdPG increased after 3 hours of treatment and Cd concentration decreased simultaneously, which was more obvious in ZnPG.Conclusion: Zn and short term low-dose Cd pre-treatment might reduce the adverse effects of Cd by increasing expression of Mts genes in SERTOLI CELLS. The protective effect of Zn was stronger than Cd.

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    APA: Copy

    KHERADMAND, FATEMEH, NOURMOHAMMADI, ISSA, AHMADI FAGHIH, MOHAMMAD AMIN, FIROOZRAI, MOHSEN, & MODARRESSI, MOHAMMAD HOSSEIN. (2013). ZINC AND LOW-DOSE OF CADMIUM PROTECT SERTOLI CELLS AGAINST TOXIC-DOSE OF CADMIUM: THE ROLE OF METALLOTHIONEIN. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), 11(6), 487-494. SID. https://sid.ir/paper/296072/en

    Vancouver: Copy

    KHERADMAND FATEMEH, NOURMOHAMMADI ISSA, AHMADI FAGHIH MOHAMMAD AMIN, FIROOZRAI MOHSEN, MODARRESSI MOHAMMAD HOSSEIN. ZINC AND LOW-DOSE OF CADMIUM PROTECT SERTOLI CELLS AGAINST TOXIC-DOSE OF CADMIUM: THE ROLE OF METALLOTHIONEIN. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE)[Internet]. 2013;11(6):487-494. Available from: https://sid.ir/paper/296072/en

    IEEE: Copy

    FATEMEH KHERADMAND, ISSA NOURMOHAMMADI, MOHAMMAD AMIN AHMADI FAGHIH, MOHSEN FIROOZRAI, and MOHAMMAD HOSSEIN MODARRESSI, “ZINC AND LOW-DOSE OF CADMIUM PROTECT SERTOLI CELLS AGAINST TOXIC-DOSE OF CADMIUM: THE ROLE OF METALLOTHIONEIN,” INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), vol. 11, no. 6, pp. 487–494, 2013, [Online]. Available: https://sid.ir/paper/296072/en

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