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Information Journal Paper

Title

EFFECT OF CAFFEINE ON MOTILITY AND VITALITY OF SPERM AND IN VITRO FERTILIZATION OF OUTBREED MOUSE IN T6 AND M16 MEDIA

Pages

  741-746

Abstract

 Background: CAFFEINE increases the CAMP production that stimulates SPERMATOZOA movement. CAFFEINE is also used for induction of in vitro acrosome reaction in mammalian SPERMATOZOA, an important step in achieving fertilization.Objective: The aim of this study was to assess the effect of CAFFEINE on sperm's motility, vitality and laboratory fertilization rates in mouse in two T6 and M16 media.Materials and Methods: Epididymal mouse sperms were collected and treated by caffine in T6 and M16 media and their motility and vitality rates were evaluated. The pretreated sperms were added to oocytes in T6 and M16 media with and without CAFFEINE and fertilization rates were recorded after 24 hours incubation.Results: Sperm's motility (81.7±1.67%) and vitality (88.7±1.33%) rates and percentage of fertilized oocytes (67.52±8.16%) in T6 medium plus CAFFEINE compare to control group have increased and shown significant differences at p≤0.01. While the percentages of these parameters in M16 medium supplemented with CAFFEINE were 68.3±6.01%, 78±6.11%, and 42.6±12.96 respectively and in comparison to control group (M16 without CAFFEINE) have not shown significant differences.Conclusion: Addition of CAFFEINE to T6 medium promotes the sperm's motility and vitality and enhances fertilization and early in vitro development of mouse embryos.

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    APA: Copy

    NABAVI, NARGES, TODEHDEHGHAN, FATEMEH, & SHIRAVI, ABDOLLHOSSEIN. (2013). EFFECT OF CAFFEINE ON MOTILITY AND VITALITY OF SPERM AND IN VITRO FERTILIZATION OF OUTBREED MOUSE IN T6 AND M16 MEDIA. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), 11(9), 741-746. SID. https://sid.ir/paper/296140/en

    Vancouver: Copy

    NABAVI NARGES, TODEHDEHGHAN FATEMEH, SHIRAVI ABDOLLHOSSEIN. EFFECT OF CAFFEINE ON MOTILITY AND VITALITY OF SPERM AND IN VITRO FERTILIZATION OF OUTBREED MOUSE IN T6 AND M16 MEDIA. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE)[Internet]. 2013;11(9):741-746. Available from: https://sid.ir/paper/296140/en

    IEEE: Copy

    NARGES NABAVI, FATEMEH TODEHDEHGHAN, and ABDOLLHOSSEIN SHIRAVI, “EFFECT OF CAFFEINE ON MOTILITY AND VITALITY OF SPERM AND IN VITRO FERTILIZATION OF OUTBREED MOUSE IN T6 AND M16 MEDIA,” INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), vol. 11, no. 9, pp. 741–746, 2013, [Online]. Available: https://sid.ir/paper/296140/en

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