ALTERNATION OF APOPTOTIC AND IMPLANTING GENES EXPRESSION OF MOUSE EMBRYOS AFTER RE-VITRIFICATION

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MAJIDI GHARENAZ NASRIN; MOVAHEDIN MANSOUREH; MAZAHERI ZOHREH; POUR BEIRANVAND SHAHRAM

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: International Journal of Reproductive BioMedicine Year:2016 | Volume:14 | Issue:8 Page(s): 511-518

Download Full-Text

View:

282

Download:

Cites:

Information Journal Paper

Title

ALTERNATION OF APOPTOTIC AND IMPLANTING GENES EXPRESSION OF MOUSE EMBRYOS AFTER RE-VITRIFICATION

Author(s)

MAJIDI GHARENAZ NASRIN | MOVAHEDIN MANSOUREH | MAZAHERI ZOHREH | POUR BEIRANVAND SHAHRAM | Issue Writer Certificate

Keywords

VITRIFICATION

EMBRYO

APOPTOSIS

GENE

Abstract

Background: Nowadays, oocytes and EMBRYOs VITRIFICATION has become a routine technique. Based on clinical judgment, re-VITRIFICATION maybe required. But little is known about re-VITRIFICATION impact on GENEs expression.Objective: The impact of re-VITRIFICATION on apoptotic and implanting GENEs, Bax, Bcl-2 and ErbB4, at compaction stage EMBRYOs were evaluated in this study.Materials and Methods: In this experimental study, 8 cell EMBRYOs (n=240) were collected from female mature mice, 60-62 hr post HCG injection. The EMBRYOs were divided randomly to 3 groups included: fresh (n=80), vitrified at 8 cell stage (n=80), vitrified at 8 cell stage thawed and re-vitrified at compaction stage (n=80). EMBRYOs were vitrified by using cryolock, (open system) described by Kuwayama. Q-PCR was used to examine the expression of Bax, Bcl2 ErbB4 GENEs in derived blastocysts.Results: Our result showed that expanded blastocyst rate was similar between vitrified and re-vitrified groups, while re-vitrified EMBRYOs showed significant decrease in expanded blastocyst rate comparing with fresh EMBRYOs (p=0.03). In addition, significant difference was observed on apoptotic GENE expression when comparing re-vitrified and fresh EMBRYOs (p=0.004), however expression of Bax and Bcl-2 (apoptotic) GENEs didn' t demonstrate a significant difference between re-vitrified and vitrified groups. The expression rate of ErbB4, an implantation GENE was decreased in re-vitrified EMBRYOs comparing with fresh EMBRYOs (p=0.003), but it was similar between re-vitrified and vitrified EMBRYOs.Conclusion: Re-VITRIFICATION can alter the expression of Bax, Bcl-2 and ErbB4 GENEs and developmental rate of mouse EMBRYOs in compaction stage.

Cites

No record.

References

No record.

Cite

APA: Copy

MAJIDI GHARENAZ, NASRIN, MOVAHEDIN, MANSOUREH, MAZAHERI, ZOHREH, & POUR BEIRANVAND, SHAHRAM. (2016). ALTERNATION OF APOPTOTIC AND IMPLANTING GENES EXPRESSION OF MOUSE EMBRYOS AFTER RE-VITRIFICATION. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), 14(8), 511-518. SID. https://sid.ir/paper/296315/en

Vancouver: Copy

MAJIDI GHARENAZ NASRIN, MOVAHEDIN MANSOUREH, MAZAHERI ZOHREH, POUR BEIRANVAND SHAHRAM. ALTERNATION OF APOPTOTIC AND IMPLANTING GENES EXPRESSION OF MOUSE EMBRYOS AFTER RE-VITRIFICATION. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE)[Internet]. 2016;14(8):511-518. Available from: https://sid.ir/paper/296315/en

IEEE: Copy

NASRIN MAJIDI GHARENAZ, MANSOUREH MOVAHEDIN, ZOHREH MAZAHERI, and SHAHRAM POUR BEIRANVAND, “ALTERNATION OF APOPTOTIC AND IMPLANTING GENES EXPRESSION OF MOUSE EMBRYOS AFTER RE-VITRIFICATION,” INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), vol. 14, no. 8, pp. 511–518, 2016, [Online]. Available: https://sid.ir/paper/296315/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops