THE EFFECT OF SUPERNATANT PRODUCT OF ADIPOSE TISSUE DERIVED MESENCHYMAL STEM CELLS AND DENSITY GRADIENT CENTRIFUGATION PREPARATION METHODS ON PREGNANCY IN INTRAUTERINE INSEMINATION CYCLES: AN RCT

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FAZAELI HODA; DAVOODI FAEZEH; KALHOR NASER; TABATABAII QOMI REZA

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Journal Paper

Paper Information

Journal: International Journal of Reproductive BioMedicine Year:2018 | Volume:16 | Issue:3 Page(s): 199-208

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Title

THE EFFECT OF SUPERNATANT PRODUCT OF ADIPOSE TISSUE DERIVED MESENCHYMAL STEM CELLS AND DENSITY GRADIENT CENTRIFUGATION PREPARATION METHODS ON PREGNANCY IN INTRAUTERINE INSEMINATION CYCLES: AN RCT

Author(s)

FAZAELI HODA | DAVOODI FAEZEH | KALHOR NASER | TABATABAII QOMI REZA | Issue Writer Certificate

Keywords

SPAS

PREPARATION METHOD

PREGNANCY

IUI

Abstract

Background: One of the most important involved factors in PREGNANCY occurrence following intrauterine insemination (IUI) is semen sample preparation. Recently, supernatant product of adipose tissue derived mesenchymal stem cells (SPAS) method has been shown to improve semen parameters.Objective: To compare the effect of PREPARATION METHODs in order to IUI, SPAS and density gradient centrifugation (DGC).Materials and Methods: This trial was done on 80 couples with male factor infertility who attend jihad daneshgahi infertility treatment center of Qom province, undergoing ovarian stimulation and IUI cycle. Various semen parameters including motility, count, DNA fragmentation and capacitation were evaluated before and after preparation. The effect of semen PREPARATION METHODs and influence of various semen parameters on PREGNANCY occurrence were examined.Results: The overall clinical PREGNANCY rate was 17.5% per patient with no miscarriage. The PREGNANCY rate for DGC and SPAS were 5% (2 of 40) and 30% (12 of 40) respectively. Since there is no significant difference in improving motion parameters between two groups (except recovery of total number of motile spermatozoa), it seems that these parameters alone are not sufficient to predict IUI PREGNANCY outcome whereas in samples with>25 million motile spermatozoa in inseminate, there was a clear trend for a higher PREGNANCY rate for the sample processed using SPAS.Conclusion: Considering SPAS as a new and effective method leading to provide a combination of various improved semen parameters, is expected in near future.

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APA: Copy

FAZAELI, HODA, DAVOODI, FAEZEH, KALHOR, NASER, & TABATABAII QOMI, REZA. (2018). THE EFFECT OF SUPERNATANT PRODUCT OF ADIPOSE TISSUE DERIVED MESENCHYMAL STEM CELLS AND DENSITY GRADIENT CENTRIFUGATION PREPARATION METHODS ON PREGNANCY IN INTRAUTERINE INSEMINATION CYCLES: AN RCT. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), 16(3), 199-208. SID. https://sid.ir/paper/296340/en

Vancouver: Copy

FAZAELI HODA, DAVOODI FAEZEH, KALHOR NASER, TABATABAII QOMI REZA. THE EFFECT OF SUPERNATANT PRODUCT OF ADIPOSE TISSUE DERIVED MESENCHYMAL STEM CELLS AND DENSITY GRADIENT CENTRIFUGATION PREPARATION METHODS ON PREGNANCY IN INTRAUTERINE INSEMINATION CYCLES: AN RCT. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE)[Internet]. 2018;16(3):199-208. Available from: https://sid.ir/paper/296340/en

IEEE: Copy

HODA FAZAELI, FAEZEH DAVOODI, NASER KALHOR, and REZA TABATABAII QOMI, “THE EFFECT OF SUPERNATANT PRODUCT OF ADIPOSE TISSUE DERIVED MESENCHYMAL STEM CELLS AND DENSITY GRADIENT CENTRIFUGATION PREPARATION METHODS ON PREGNANCY IN INTRAUTERINE INSEMINATION CYCLES: AN RCT,” INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), vol. 16, no. 3, pp. 199–208, 2018, [Online]. Available: https://sid.ir/paper/296340/en

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