Vitrification has detrimental effects on maturation, viability, and subcellular quality of oocytes post IVM in cancerous women: An experimental study

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MOHSENZADEH MEHDI; TABIBNEJAD NASIM; VATANPARAST MAHBOUBEH; ANBARI FATEMEH; KHALILI MOHAMMAD ALI; KARIMI ZARCHI MOJGAN

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Journal: International Journal of Reproductive BioMedicine Year:2019 | Volume:17 | Issue:3 Page(s): 175-184

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Title

Vitrification has detrimental effects on maturation, viability, and subcellular quality of oocytes post IVM in cancerous women: An experimental study

Author(s)

Keywords

IVM

Vitrification

Fertility preservation

Maturation rate

Meiotic spindle

Zona pellucida birefringence

Abstract

Background: In vitro maturation (IVM) of immature oocytes retrieved from ovarian tissue has been considered as a valuable approach for Fertility preservation in cancerous patients. Objective: To evaluate the efficacy of Vitrification on oocyte maturation, survival rates, as well as the subcellular oocyte quality post IVM. Materials and Methods: The ovarian cortexes from 19 women with cervix and uterine malignancy aged 21– 39 yr were collected. Cumulus-oocyte complexes were aspirated from all visible antral follicles. 102 immature oocytes were collected, and 43 oocytes were detected appropriately for IVM (control group). Also, 59 immature oocytes were vitrified, then matured in vitro (IVM) in two groups: with Growth/differentiation factor 9 (GDF9) (group 1) and without GDF9 (group 2) supplementation. Rates of oocytes viability, maturation, and survival along with Meiotic spindle visualization and Zona pellucida birefringence were assessed with Polyscope. Results: The rate of maturation was significantly higher in controls (55. 8%) compared to the other groups. Maturation rate was 23. 3% in oocytes cultured in IVM medium enriched with GDF9, and 27. 6% in those cultured in IVM medium lacking GDF9 (p = 0. 86). Also, the Meiotic spindle was present in 74. 4% of control oocytes which was significantly higher than the other groups. The proportion of high Zona pellucida birefringence was higher in the controls when compared with group 1 (51. 2% vs. 23. 3%, respectively, p = 0. 04). Conclusion: Vitrification had a detrimental effect on oocyte maturation, viability as well as the subcellular quality of the oocytes after IVM in cancerous women.

Cites

Optimization of Ovine-Oocyte Vitrification Utilizing Calcium Depletion by Adding EGTA to Freezing Solution

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APA: Copy

MOHSENZADEH, MEHDI, TABIBNEJAD, NASIM, VATANPARAST, MAHBOUBEH, ANBARI, FATEMEH, KHALILI, MOHAMMAD ALI, & KARIMI ZARCHI, MOJGAN. (2019). Vitrification has detrimental effects on maturation, viability, and subcellular quality of oocytes post IVM in cancerous women: An experimental study. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), 17(3), 175-184. SID. https://sid.ir/paper/296359/en

Vancouver: Copy

MOHSENZADEH MEHDI, TABIBNEJAD NASIM, VATANPARAST MAHBOUBEH, ANBARI FATEMEH, KHALILI MOHAMMAD ALI, KARIMI ZARCHI MOJGAN. Vitrification has detrimental effects on maturation, viability, and subcellular quality of oocytes post IVM in cancerous women: An experimental study. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE)[Internet]. 2019;17(3):175-184. Available from: https://sid.ir/paper/296359/en

IEEE: Copy

MEHDI MOHSENZADEH, NASIM TABIBNEJAD, MAHBOUBEH VATANPARAST, FATEMEH ANBARI, MOHAMMAD ALI KHALILI, and MOJGAN KARIMI ZARCHI, “Vitrification has detrimental effects on maturation, viability, and subcellular quality of oocytes post IVM in cancerous women: An experimental study,” INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), vol. 17, no. 3, pp. 175–184, 2019, [Online]. Available: https://sid.ir/paper/296359/en

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