Upstream or swim up processing technique: which one is more effective to select human sperm with high chromatin integrity (Short communication)

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HEIDARI MAHNAZ; LAKPOUR NIKNAM; DARBANDI MAHSA; Darbani Sara; SHANI SAEIDEH; GOHARBAKHSH LEILA; Cheshmi Ghazaleh; AKHONDI MOHAMMAD MEHDI; SADEGHI MOHAMMAD REZA

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Journal: International Journal of Reproductive BioMedicine Year:2018 | Volume:16 | Issue:7 Page(s): 463-468

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Title

Upstream or swim up processing technique: which one is more effective to select human sperm with high chromatin integrity (Short communication)

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Keywords

Chromatin maturation

DNA fragmentation

Sperm

Swim up

Upstream technique

Abstract

Background: Sperm processing methods separate motile Sperms with good morphology from dead and abnormal forms of Sperms, immature germ cells, and non-Sperm cells. Objective: The propose of this study was to compare the efficacy of upstream and swim-up processing techniques to separate Sperms with the high quality especially in relation to Sperm chromatin integrity. Materials and Methods: This experimental study used semen samples from 60 normozooSpermic men. Specimens were divided into equal aliquots for processing by Swim up (group A), and upstream (group B) methods and compare with control by raw semen (group C). Sperm concentration, morphology, motility, DNA fragmentation and Chromatin maturation were measured in these three groups. Results: The results revealed that Sperm concentration in the Swim up samples was significantly greater than upstream samples (p≤ 0. 04). as addition, motile Sperm recovery including the percentage of progressive motility and a total number of motile Sperm was better in the swim-up compared to an upstream method and raw semen (p≤ 0. 001). The cell debris and seminal fluid were equally removed by both methods and the percentage of normal forms was also similar in both procedures (p≥ 0. 4). In addition, Sperm DNA fragmentation and Chromatin maturation were not significantly different between the three groups (p≥ 0. 1). Conclusion: According to results, apparently the upstream method had no significant efficiency to separate good quality Sperms compare to Swim up. Therefore, Swim up seems to be a simple, inexpensive, reliable and widely available method with an efficient yield to separate motile Sperm with good morphology and better chromatin integrity for insemination in the infertility clinics.

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APA: Copy

HEIDARI, MAHNAZ, LAKPOUR, NIKNAM, DARBANDI, MAHSA, Darbani, Sara, SHANI, SAEIDEH, GOHARBAKHSH, LEILA, Cheshmi, Ghazaleh, AKHONDI, MOHAMMAD MEHDI, & SADEGHI, MOHAMMAD REZA. (2018). Upstream or swim up processing technique: which one is more effective to select human sperm with high chromatin integrity (Short communication). INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), 16(7), 463-468. SID. https://sid.ir/paper/296511/en

Vancouver: Copy

HEIDARI MAHNAZ, LAKPOUR NIKNAM, DARBANDI MAHSA, Darbani Sara, SHANI SAEIDEH, GOHARBAKHSH LEILA, Cheshmi Ghazaleh, AKHONDI MOHAMMAD MEHDI, SADEGHI MOHAMMAD REZA. Upstream or swim up processing technique: which one is more effective to select human sperm with high chromatin integrity (Short communication). INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE)[Internet]. 2018;16(7):463-468. Available from: https://sid.ir/paper/296511/en

IEEE: Copy

MAHNAZ HEIDARI, NIKNAM LAKPOUR, MAHSA DARBANDI, Sara Darbani, SAEIDEH SHANI, LEILA GOHARBAKHSH, Ghazaleh Cheshmi, MOHAMMAD MEHDI AKHONDI, and MOHAMMAD REZA SADEGHI, “Upstream or swim up processing technique: which one is more effective to select human sperm with high chromatin integrity (Short communication),” INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), vol. 16, no. 7, pp. 463–468, 2018, [Online]. Available: https://sid.ir/paper/296511/en

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