مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

248
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

129
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

2

Information Journal Paper

Title

Supplementation of L-carnitine during in vitro maturation of mouse oocytes affects expression of genes involved in oocyte and embryo competence: An experimental study

Pages

  779-786

Abstract

 Background: Oocyte developmental competence is one of the key factors for determining the success rate of assisted reproductive technique. Objective: The aim of the current study was to investigate the effect of L-carnitine (LC) supplementation during in vitro maturation (IVM), on preimplantation embryo development and expression of genes involved in embryo competence derived from Oocytes selected with brilliant cresyl blue (BCB) test. Materials and Methods: Cumulus-Oocyte complexes (COCs) were obtained from NMRI mice ovaries. COCs were stained with BCB and then BCB+ (colored cytoplasm) Oocytes cultured in IVM medium supplemented with 0. 3 or 0. 6 mg/ml LC. COCs untreated with LC were used as control. Fertilization rate and blastocyst development rate were determined after In vitro fertilization. In addition, quantitative reverse transcriptase polymerase chain reaction was used to measure relative genes expression related with development (Ccnb1, Mos, Ces5, and Dppa2) and apoptosis (Bax and Bcl-xL) in Oocytes and embryos. Results: Oocytes treated with both LC concentrations showed higher blastocyst development rate compared with untreated Oocytes (p<0. 01). Moreover, fertilization rate was increased in Oocytes treated with 0. 6 mg/ml LC (p<0. 01). Treatment of Oocytes with both LC concentrations increased (p<0. 01) the level of Ccnb1 mRNA in MII Oocytes. The two-cell stage embryos and blastocysts derived from LC-treated Oocytes (0. 6 mg/ml) showed increased the expression levels of Dppa2 and Bcl-xl mRNA, respectively (p<0. 01). Conclusion: The results of the present study show that adding of LC to the IVM medium of BCB+ Oocytes can ameliorate reproductive success following In vitro fertilization.

Cites

References

  • No record.

    • Cite

    APA: Copy

    Zare, Zohreh, ABOUHAMZEH, BEHESHTEH, MASTERI FARAHANI, REZA, SALEHI, MOHAMMAD, & MOHAMMADI, MOSLEM. (2017). Supplementation of L-carnitine during in vitro maturation of mouse oocytes affects expression of genes involved in oocyte and embryo competence: An experimental study. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), 15(12), 779-786. SID. https://sid.ir/paper/296518/en

    Vancouver: Copy

    Zare Zohreh, ABOUHAMZEH BEHESHTEH, MASTERI FARAHANI REZA, SALEHI MOHAMMAD, MOHAMMADI MOSLEM. Supplementation of L-carnitine during in vitro maturation of mouse oocytes affects expression of genes involved in oocyte and embryo competence: An experimental study. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE)[Internet]. 2017;15(12):779-786. Available from: https://sid.ir/paper/296518/en

    IEEE: Copy

    Zohreh Zare, BEHESHTEH ABOUHAMZEH, REZA MASTERI FARAHANI, MOHAMMAD SALEHI, and MOSLEM MOHAMMADI, “Supplementation of L-carnitine during in vitro maturation of mouse oocytes affects expression of genes involved in oocyte and embryo competence: An experimental study,” INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), vol. 15, no. 12, pp. 779–786, 2017, [Online]. Available: https://sid.ir/paper/296518/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button