CD8+ T Lymphocyte Subsets in Bladder Tumor Draining Lymph Nodes

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Faghih Zahra; Shobeiri Saeideh Sadat; ARIAFAR ALI; Sarkarian Mohsen; ZEIGHAMI SHAHRYAR; Nazari Nazanin; Abbasi Sarvak Saeed; ERFANI NASROLLAH

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Journal: Iranian Journal of Immunology Year:2016 | Volume:13 | Issue:4 Page(s): 237-248

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Title

CD8+ T Lymphocyte Subsets in Bladder Tumor Draining Lymph Nodes

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Keywords

Bladder Cancer

Lymph Node

CD8+ lymphocytes

Tc1

Tc2

Tc17

Abstract

Background: Cytotoxic CD8+ T cells, as essential parts of the adaptive immune system, play pivotal roles in anti-tumor immune responses. It is well documented that cytokine expression profiles and activation status of these cells during anti-tumor immune responses affect the outcome of host-tumor interaction. Objective: To investigate the percentages of CD8+ lymphocytes and their subsets in tumor draining Lymph Nodes of patients with Bladder Cancer. Methods: Forty-five patients with Bladder Cancer, candidate for radical cystectomy, were recruited. Mononuclear cells were isolated from draining Lymph Nodes using Ficoll-Hypaque gradient centrifugation, and were activated by PMA/Ionomycin in the presence of Golgi inhibitors. The cells were then permeabilized and stained with appropriate flourochrome conjugated antibodies against CD3, CD8, IFN-γ , IL-17 and IL-4 molecules. Data were collected on a fourcolor flow cytometer and analyzed by CellQuestPro software. Results: Despite no difference in the frequency of IL-17 producing CD8+ (Tc17) lymphocytes, the mean expression of IL-17 in this subset was significantly elevated in high-grade patients (p=0. 011). The percentage of double positive IFN-γ /IL-17 CD8+ lymphocytes was also significantly increased in node positive patients compared to node negative ones (p=0. 046). Our results also demonstrated that the percentage of IFN-γ producing CD8+ (Tc1) lymphocytes was significantly increased in the patients with higher histological grade compared to those with lower ones (p=0. 038). Conclusion: IFN-γ and IL-17 producing CD8+ T cells may increase in advanced stages of Bladder Cancer, but their correlation with tumor prognosis remains to be investigated.

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APA: Copy

Faghih, Zahra, Shobeiri, Saeideh Sadat, ARIAFAR, ALI, Sarkarian, Mohsen, ZEIGHAMI, SHAHRYAR, Nazari, Nazanin, Abbasi Sarvak, Saeed, & ERFANI, NASROLLAH. (2016). CD8+ T Lymphocyte Subsets in Bladder Tumor Draining Lymph Nodes. IRANIAN JOURNAL OF IMMUNOLOGY (IJI), 13(4), 237-248. SID. https://sid.ir/paper/296926/en

Vancouver: Copy

Faghih Zahra, Shobeiri Saeideh Sadat, ARIAFAR ALI, Sarkarian Mohsen, ZEIGHAMI SHAHRYAR, Nazari Nazanin, Abbasi Sarvak Saeed, ERFANI NASROLLAH. CD8+ T Lymphocyte Subsets in Bladder Tumor Draining Lymph Nodes. IRANIAN JOURNAL OF IMMUNOLOGY (IJI)[Internet]. 2016;13(4):237-248. Available from: https://sid.ir/paper/296926/en

IEEE: Copy

Zahra Faghih, Saeideh Sadat Shobeiri, ALI ARIAFAR, Mohsen Sarkarian, SHAHRYAR ZEIGHAMI, Nazanin Nazari, Saeed Abbasi Sarvak, and NASROLLAH ERFANI, “CD8+ T Lymphocyte Subsets in Bladder Tumor Draining Lymph Nodes,” IRANIAN JOURNAL OF IMMUNOLOGY (IJI), vol. 13, no. 4, pp. 237–248, 2016, [Online]. Available: https://sid.ir/paper/296926/en

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