Designing and Expression of Recombinant Chimeric Protein Containing CtxB and OmpW from Vibrio Cholerae and Evaluation of Its Immunogenicity

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Vakili Atina; MOUSAVI GARGARI SEYED LATIF; NAZARIAN SHAHRAM; AMANI JAFAR

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Journal of Immunology Year:2018 | Volume:15 | Issue:3 Page(s): 207-220

Download Full-Text

View:

169

Download:

161

Cites:

Information Journal Paper

Title

Designing and Expression of Recombinant Chimeric Protein Containing CtxB and OmpW from Vibrio Cholerae and Evaluation of Its Immunogenicity

Author(s)

Vakili Atina | MOUSAVI GARGARI SEYED LATIF | NAZARIAN SHAHRAM | AMANI JAFAR | Issue Writer Certificate

Keywords

Bioinformatics

CtxB

Immunization

OmpW

Recombinant Protein

Vibrio Cholerae

Abstract

Background: Cholera disease caused by Vibrio Cholerae remains a major cause of morbidity and mortality throughout the world. Various strategies with different proteins as immunogens have been tried for vaccine development, none of which have been sufficiently effective to preclude cholera. Chimeric proteins, with their ability to present multiple antigens at the same time, can play important roles in Immunization. Objective: To evaluate the immunogenicity of a chimeric construct, comprised of OmpW and CtxB as immunogenic proteins of Vibrio cholera, in BALB/c mice. Methods: The construct was designed after Bioinformatics assessments and then expressed in E. coli. Chimeric protein, OmpW, and CtxB were purified with Ni-NTA chromatography and confirmed by Western blotting. Mice were immunized with purified Recombinant Proteins. The antibody titers and specificity of the immune sera were then analyzed by ELISA and challenged on the pups of immunized mice with 1, 5 and 10 LD50. Mice ileal loop assay was also performed. Results: Significant differences were observed in antibody titers in immunized mice compared to the control groups. Infant mouse challenge was performed so as to compare the protective efficacies of the selected immunogen regimens. Of the Pups from dams immunized with chimeric protein which received 1 LD50, 75% survived. Pups belonging to PBS-immunized dams, experienced 100% mortality. The serum raised toward immunogenic construct, inhibited cholera toxin activity in ileal loop test up to 68%. Conclusion: Chimeric construct is able to induce the immune system and provide up to 75% inhibition of toxin activity against 1 LD50 of Vibrio Cholerae.

Cites

No record.

References

No record.

Cite

APA: Copy

Vakili, Atina, MOUSAVI GARGARI, SEYED LATIF, NAZARIAN, SHAHRAM, & AMANI, JAFAR. (2018). Designing and Expression of Recombinant Chimeric Protein Containing CtxB and OmpW from Vibrio Cholerae and Evaluation of Its Immunogenicity. IRANIAN JOURNAL OF IMMUNOLOGY (IJI), 15(3), 207-220. SID. https://sid.ir/paper/296954/en

Vancouver: Copy

Vakili Atina, MOUSAVI GARGARI SEYED LATIF, NAZARIAN SHAHRAM, AMANI JAFAR. Designing and Expression of Recombinant Chimeric Protein Containing CtxB and OmpW from Vibrio Cholerae and Evaluation of Its Immunogenicity. IRANIAN JOURNAL OF IMMUNOLOGY (IJI)[Internet]. 2018;15(3):207-220. Available from: https://sid.ir/paper/296954/en

IEEE: Copy

Atina Vakili, SEYED LATIF MOUSAVI GARGARI, SHAHRAM NAZARIAN, and JAFAR AMANI, “Designing and Expression of Recombinant Chimeric Protein Containing CtxB and OmpW from Vibrio Cholerae and Evaluation of Its Immunogenicity,” IRANIAN JOURNAL OF IMMUNOLOGY (IJI), vol. 15, no. 3, pp. 207–220, 2018, [Online]. Available: https://sid.ir/paper/296954/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops