مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Information Journal Paper

Title

Antitumor Immunity Induced by Genetic Immunization with Chitosan Nanoparticle Formulated Adjuvanted for HPV-16 E7 DNA Vaccine

Pages

  269-280

Abstract

 Background: In recent years attention has been paid to develop effective adjuvant systems for DNA vaccines. Co-formulation of a gene delivery vector with an immunostimulator can enhance therapeutic efficiency of DNA vaccine. Objective: To investigate the efficacy of chitosan as a nanodelivery system to enhance antiTumor effects of human papilloma virus (HPV)-16 DNA vaccine with IL-12 gene for protection against TC-1 Tumor using an animal model. Methods: The mice were challenged by subcutaneous injection of TC-1 cells and immunized intramuscularly with DNA vaccine thrice at seven-day intervals. One week after the last immunization, mice were sacrificed and antiTumor effects were assessed through measuring lymphocyte proliferation, cytotoxicity, cytokines production, and Tumor regression. Results: We found that co-formulation and co-administration of Chitosan nanoparticles and IL-12 with HPV-16 E7 DNA vaccine induced higher antiTumor effects compared with chitosan or IL-12 alone. E7-specific lymphocyte proliferation index and CTL activity were found to be significantly higher in combination group in comparison to single vaccination with either chitosan or IL-12. Co-formulation of chitosan and IL-12 resulted in higher IFN-γ and IL-4, and decreased IL-10 production. Furthermore, combined vaccination highly inhibited the Tumor progression compared with chitosan or IL-12 alone. Conclusion: Chitosan nanoparticle is a promising delivery system for DNA vaccine and IL-12 is an effective genetic adjuvant for the induction of strong antiTumor immune response.

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    APA: Copy

    TAHAMTAN, ALIREZA, BARATI, MOHAMMAD, TABARRAEI, ALIJAN, MOHEBBI, SEYED REZA, SHIRIAN, SADEGH, GORJI, ALI, & GHAEMI, AMIR. (2018). Antitumor Immunity Induced by Genetic Immunization with Chitosan Nanoparticle Formulated Adjuvanted for HPV-16 E7 DNA Vaccine. IRANIAN JOURNAL OF IMMUNOLOGY (IJI), 15(4), 269-280. SID. https://sid.ir/paper/296961/en

    Vancouver: Copy

    TAHAMTAN ALIREZA, BARATI MOHAMMAD, TABARRAEI ALIJAN, MOHEBBI SEYED REZA, SHIRIAN SADEGH, GORJI ALI, GHAEMI AMIR. Antitumor Immunity Induced by Genetic Immunization with Chitosan Nanoparticle Formulated Adjuvanted for HPV-16 E7 DNA Vaccine. IRANIAN JOURNAL OF IMMUNOLOGY (IJI)[Internet]. 2018;15(4):269-280. Available from: https://sid.ir/paper/296961/en

    IEEE: Copy

    ALIREZA TAHAMTAN, MOHAMMAD BARATI, ALIJAN TABARRAEI, SEYED REZA MOHEBBI, SADEGH SHIRIAN, ALI GORJI, and AMIR GHAEMI, “Antitumor Immunity Induced by Genetic Immunization with Chitosan Nanoparticle Formulated Adjuvanted for HPV-16 E7 DNA Vaccine,” IRANIAN JOURNAL OF IMMUNOLOGY (IJI), vol. 15, no. 4, pp. 269–280, 2018, [Online]. Available: https://sid.ir/paper/296961/en

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