مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Information Journal Paper

Title

Multiple Myeloma Bone Marrow Mesenchymal Stromal Cells Inhibit CD8+ T Cell Function in a Process that May Implicate Fibroblast Activation Protein α

Pages

  278-290

Abstract

 Background: Multiple Myeloma (MM) is a malignant plasma cell proliferative disorder with limited immunotherapy treatment because of T cell dysfunction. Objective: To investigate the immunomodulatory function of Bone Marrow Mesenchymal Stromal Cells (MM-BMSCs) on CD8+ T cells. Methods: Proliferation and cytotoxicity were detected by cell counting kit-8 assay. Cell cycle was detected by flow cytometry, and p16 expression was detected by PCR. The expression of fibroblast activation protein α (FAPα ) was evaluated by immunohistochemistry. Results: Co-culture of CD8+ T cells with MM-BMSCs decreased the cell survival rate and increased the killing rate (p=0. 03, p=0. 001, respectively), the percentage of cells in G0/G1 phase and p16 expression (p<0. 001). FAPα was mainly in the mesenchymal matrix of the MM microenvironment and elevated in MM derived bone marrow compared to healthy donors (p<0. 001). The FAPα inhibitor PT-100, increased survival and the killing rate (p<0. 001, p=0. 043, respectively), and decreased the percentage of cells in G0/G1 phase and p16 expression (p=0. 024, p=0. 004, respectively). Conclusion: Therefore, MM-BMSCs inhibit the proliferation and cytotoxicity of CD8+ T cells, significantly block the cell cycle and increase p16 expression in co-cultured CD8+ T cells in a cell-cell contact-dependent manner.

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    Cite

    APA: Copy

    Wang, Yadan, Wu, Xiaofei, & Hu, Yu. (2019). Multiple Myeloma Bone Marrow Mesenchymal Stromal Cells Inhibit CD8+ T Cell Function in a Process that May Implicate Fibroblast Activation Protein α. IRANIAN JOURNAL OF IMMUNOLOGY (IJI), 16(4), 278-290. SID. https://sid.ir/paper/296974/en

    Vancouver: Copy

    Wang Yadan, Wu Xiaofei, Hu Yu. Multiple Myeloma Bone Marrow Mesenchymal Stromal Cells Inhibit CD8+ T Cell Function in a Process that May Implicate Fibroblast Activation Protein α. IRANIAN JOURNAL OF IMMUNOLOGY (IJI)[Internet]. 2019;16(4):278-290. Available from: https://sid.ir/paper/296974/en

    IEEE: Copy

    Yadan Wang, Xiaofei Wu, and Yu Hu, “Multiple Myeloma Bone Marrow Mesenchymal Stromal Cells Inhibit CD8+ T Cell Function in a Process that May Implicate Fibroblast Activation Protein α,” IRANIAN JOURNAL OF IMMUNOLOGY (IJI), vol. 16, no. 4, pp. 278–290, 2019, [Online]. Available: https://sid.ir/paper/296974/en

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