Effect of Tribulus Terrestris L. on Expression of ICAM-1, VCAM-1, E-Selectin and Proteome Profile of Human Endothelial Cells In-Vitro

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Amirinezhad Fard Elahe; FEREYDOUNI ZAHRA; MANSOURI KAMRAN; MOSTAFAIE ALI

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Journal: Iranian Journal of Immunology Year:2020 | Volume:17 | Issue:1 Page(s): 64-74

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Information Journal Paper

Title

Effect of Tribulus Terrestris L. on Expression of ICAM-1, VCAM-1, E-Selectin and Proteome Profile of Human Endothelial Cells In-Vitro

Author(s)

Amirinezhad Fard Elahe | FEREYDOUNI ZAHRA | MANSOURI KAMRAN | MOSTAFAIE ALI | Issue Writer Certificate

Keywords

ICAM-1

VCAM-1

Endothelial Cells

Proteome

Tribulus Terrestris

Abstract

Background: Atherosclerosis is a chronic inflammation that interferes with blood arteries functions due to the accumulation of low density lipids and cholesterol. Objective: To investigate the effect of aqueous extract and saponin fraction of Tribulus Terrestris L. (TT) on the Proteome and expression of intracellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin in the human umbilical vein Endothelial Cells (HUVEC) and human bone marrow endothelial cell (HBMEC) lines. Methods: Two cell lines were cultured and induced with lipopolysaccharide (LPS). The primed cells were then treated with aqueous extract and saponin fraction of TT. The protein profile of the Endothelial Cells was assessed under normal and LPS-induced conditions using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and 2D gel electrophoresis (2-DE). The levels of VCAM-1, ICAM-1, and E-selectin were estimated by use of western blotting. Results: LPS-induced HUVECs and HBMECs were shown to significantly increase the expression of ICAM-1, VCAM-1, and E-selectin in comparison to control groups. Our findings revealed that TT extract resulted in significantly more reduced levels of proteom (80 spots) as well as all the three mentioned proteins compared with the effect of saponin fraction alone. Conclusion: TT extract and its saponin fraction exerted anti-inflammatory effects on HUVEC and HBMEC lines and reduced the expression of ICAM-1, VCAM-1, and E-selectin. However, the anti-inflammatory effect of aqueous extract was greater than that of saponin fraction. Therefore, TT could be considered as a potential candidate for the treatment or prevention of atherosclerosis.

Cites

Effect of Eight Weeks of Resistance Training and Consumption of Tribulus Terrestris on Androgenic Receptor-1, Fas Ligand Gene Expression, and Lipid Profiles in Rats Exposed to Stanozolol

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APA: Copy

Amirinezhad Fard, Elahe, FEREYDOUNI, ZAHRA, MANSOURI, KAMRAN, & MOSTAFAIE, ALI. (2020). Effect of Tribulus Terrestris L. on Expression of ICAM-1, VCAM-1, E-Selectin and Proteome Profile of Human Endothelial Cells In-Vitro. IRANIAN JOURNAL OF IMMUNOLOGY (IJI), 17(1), 64-74. SID. https://sid.ir/paper/296985/en

Vancouver: Copy

Amirinezhad Fard Elahe, FEREYDOUNI ZAHRA, MANSOURI KAMRAN, MOSTAFAIE ALI. Effect of Tribulus Terrestris L. on Expression of ICAM-1, VCAM-1, E-Selectin and Proteome Profile of Human Endothelial Cells In-Vitro. IRANIAN JOURNAL OF IMMUNOLOGY (IJI)[Internet]. 2020;17(1):64-74. Available from: https://sid.ir/paper/296985/en

IEEE: Copy

Elahe Amirinezhad Fard, ZAHRA FEREYDOUNI, KAMRAN MANSOURI, and ALI MOSTAFAIE, “Effect of Tribulus Terrestris L. on Expression of ICAM-1, VCAM-1, E-Selectin and Proteome Profile of Human Endothelial Cells In-Vitro,” IRANIAN JOURNAL OF IMMUNOLOGY (IJI), vol. 17, no. 1, pp. 64–74, 2020, [Online]. Available: https://sid.ir/paper/296985/en

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