Inhibitory Effects of Antimicrobial Photodynamic Therapy with Curcumin on Biofilm-Associated Gene Expression Profile of

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POURHAJIBAGHER MARYAM; CHINIFORUSH NASIM; MONZAVI ABBAS; BARIKANI HAMIDREZA; Monzavi Mohammad Moein; Sobhani Shaghayegh; SHAHABI SIMA; BAHADOR ABBAS

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Journal: Frontiers in Dentistry Year:2018 | Volume:15 | Issue:3 Page(s): 169-177

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Title

Inhibitory Effects of Antimicrobial Photodynamic Therapy with Curcumin on Biofilm-Associated Gene Expression Profile of

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Keywords

Aggregatibacter actinomycetemcomitans

Biofilms

Curcumin

Periodontitis

Quantitative Real-Time Polymerase Chain Reaction

Abstract

Objectives: Periodontitis is an inflammation of periodontal tissues that is caused by the biofilm of periodontal pathogens. Aggregatibacter actinomycetemcomitans (A. actinomycetemcomitans) is an opportunistic periodontopathogen that can be the cause of periodontal diseases via fimbriae as a virulence factor. In this study, we aimed to determine the expression level of A. actinomycetemcomitans rcpA gene as a virulence factor associated with biofilm formation after antimicrobial photodynamic therapy (aPDT) as a relatively new therapeutic modality. Materials and Methods: To determine sub-lethal doses of aPDT against A. actinomycetemcomitans ATCC 33384 strain, we used Curcumin (CUR) as a photosensitizer at a final concentration of 40 μ mol/ml, which was excited with a light-emitting diode (LED) at the wavelength of 450 nm. Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) was then applied to monitor rcpA gene expression in A. actinomycetemcomitans. Results: 10-40 μ mol/ml of CUR caused a significant reduction in the growth of A. actinomycetemcomitans compared to control group (P<0. 05). Also, the cell viability of A. actinomycetemcomitans was significantly decreased after more than four minutes of LED irradiation. Therefore, the sub-lethal dose of aPDT against A. actinomycetemcomitans was 5 μ mol/ml of CUR with three minutes of LED irradiation at a fluency of 180-240 J/cm2, which reduced the expression of the rcpA gene by approximately 8. 5-fold. Conclusions: aPDT with CUR leads to decreased cell survival and virulence of A. actinomycetemcomitans. Thus, CUR-aPDT can be used as an alternative approach for the successful treatment of Periodontitis in vivo.

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APA: Copy

POURHAJIBAGHER, MARYAM, CHINIFORUSH, NASIM, MONZAVI, ABBAS, BARIKANI, HAMIDREZA, Monzavi, Mohammad Moein, Sobhani, Shaghayegh, SHAHABI, SIMA, & BAHADOR, ABBAS. (2018). Inhibitory Effects of Antimicrobial Photodynamic Therapy with Curcumin on Biofilm-Associated Gene Expression Profile of. FRONTIERS IN DENTISTRY (JOURNAL OF DENTISTRY OF TEHRAN UNIVERSITY OF MEDICAL SCIENCES), 15(3), 169-177. SID. https://sid.ir/paper/298143/en

Vancouver: Copy

POURHAJIBAGHER MARYAM, CHINIFORUSH NASIM, MONZAVI ABBAS, BARIKANI HAMIDREZA, Monzavi Mohammad Moein, Sobhani Shaghayegh, SHAHABI SIMA, BAHADOR ABBAS. Inhibitory Effects of Antimicrobial Photodynamic Therapy with Curcumin on Biofilm-Associated Gene Expression Profile of. FRONTIERS IN DENTISTRY (JOURNAL OF DENTISTRY OF TEHRAN UNIVERSITY OF MEDICAL SCIENCES)[Internet]. 2018;15(3):169-177. Available from: https://sid.ir/paper/298143/en

IEEE: Copy

MARYAM POURHAJIBAGHER, NASIM CHINIFORUSH, ABBAS MONZAVI, HAMIDREZA BARIKANI, Mohammad Moein Monzavi, Shaghayegh Sobhani, SIMA SHAHABI, and ABBAS BAHADOR, “Inhibitory Effects of Antimicrobial Photodynamic Therapy with Curcumin on Biofilm-Associated Gene Expression Profile of,” FRONTIERS IN DENTISTRY (JOURNAL OF DENTISTRY OF TEHRAN UNIVERSITY OF MEDICAL SCIENCES), vol. 15, no. 3, pp. 169–177, 2018, [Online]. Available: https://sid.ir/paper/298143/en

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