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Information Journal Paper

Title

Simultaneous Estimation of Amlodipine Besylate and Nebivolol Hydrochloride in Pharmaceutical Tablets Formulation by RP-HPLC Using PDA Detector

Pages

  45-56

Abstract

 The present work was undertaken with the aim to develop and validate a rapid and consistent RP-HPLC method in which the peaks will be appear with short period of time as per ICH Guidelines. The separation was achieved on a stainless steel analytical column, Eclipse XDB plus C18 column (4. 6 X 150 mm; 5 μ m) in an isocratic mode. The mobile phase was composed acetonitrile and 0. 01 M ammonium acetate (pH adjusted to 4. 5 using glacial acetic acid), which were mixed in the ratio of 50: 50. The flow rate was monitored at 1. 0 mL/min. The wavelength selected for detection was 265 nm. The retention time found for Amlodipine besylate and nebivolol hydrochloride was 2. 967 and 3. 510 min, respectively. The % recovery was 100. 20-100. 86 for amlodipine and 100. 20-100. 78 for nebivolol. The linearity was established in the range of 5-25 μ g/mL for amlodipine and 10-50 μ g/mL for nebivolol. The slope, intercept, and correlation coefficient were found to be 314. 2x, +162. 4, and 0. 999 for Amlodipine besylate and 248x,-305. 7, and 0. 9998 for nebivolol hydrochloride, respectively. The limits of detection for Amlodipine besylate and nebivolol hydrochloride obtained by the proposed method was 0. 07 and 0. 20 μ g/ml and the limits of quantification for Amlodipine besylate and nebivolol hydrochloride obtained by the proposed method was 0. 23 and 0. 61 μ g/mL, respectively. The method was found to be suitable for the quality control test of Amlodipine besylate and nebivolol hydrochloride simultaneously in a bulk drugs as well as in a formulations.

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    • Cite

    APA: Copy

    Kumar, Ashutosh, Debnath, Manidipa, Sreenu, Mendeli, & Kumar Singh, Mahesh. (2018). Simultaneous Estimation of Amlodipine Besylate and Nebivolol Hydrochloride in Pharmaceutical Tablets Formulation by RP-HPLC Using PDA Detector. IRANIAN JOURNAL OF PHARMACEUTICAL SCIENCES, 14(1), 45-56. SID. https://sid.ir/paper/300381/en

    Vancouver: Copy

    Kumar Ashutosh, Debnath Manidipa, Sreenu Mendeli, Kumar Singh Mahesh. Simultaneous Estimation of Amlodipine Besylate and Nebivolol Hydrochloride in Pharmaceutical Tablets Formulation by RP-HPLC Using PDA Detector. IRANIAN JOURNAL OF PHARMACEUTICAL SCIENCES[Internet]. 2018;14(1):45-56. Available from: https://sid.ir/paper/300381/en

    IEEE: Copy

    Ashutosh Kumar, Manidipa Debnath, Mendeli Sreenu, and Mahesh Kumar Singh, “Simultaneous Estimation of Amlodipine Besylate and Nebivolol Hydrochloride in Pharmaceutical Tablets Formulation by RP-HPLC Using PDA Detector,” IRANIAN JOURNAL OF PHARMACEUTICAL SCIENCES, vol. 14, no. 1, pp. 45–56, 2018, [Online]. Available: https://sid.ir/paper/300381/en

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