CYTOTOXIC EFFECT OF A NEW ENDODONTIC CEMENT AND MINERAL TRIOXIDE AGGREGATE ON L929 LINE CULTURE

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GHODOUSI JAMILEH; TAVAKOL AFSHARI J.; DONYAVI Z.; BOROUK A.; DISFANI R.; ESMAEILZADEH MOHAMMAD

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Journal Paper

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Journal: Iranian Endodontic Journal Year:2008 | Volume:3 | Issue:2 Page(s): 17-23

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Title

CYTOTOXIC EFFECT OF A NEW ENDODONTIC CEMENT AND MINERAL TRIOXIDE AGGREGATE ON L929 LINE CULTURE

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Keywords

CYTOTOXICITY TEST

FIBROBLAST

NEW MATERIAL

MINERAL TRIOXIDE AGGREGATE

Abstract

Introduction: The aim of this study was to compare the cytotoxicity of MINERAL TRIOXIDE AGGREGATE (MTA) and a New Endodontic Cement (NEC) on L929 mouse FIBROBLASTs.Materials and Methods: Different dilutions (Neat, 1/2, 1/10, 1/100) of fresh and set materials placed adjacent flasks of L929 in DMEM medium. Cellular viability was assessed using MTT assay in three time intervals (24, 48, and 72 h after mixing). Differences in mean cell viability values between materials were assessed by using the One-way ANOVA and Bonferoni post-test. Optical microscopic analysis of morphology of the untreated control and the cement treated cell cultures were carried out in all experimental periods.Results: It was indicated that there was not a significant difference in cytotoxicity among the materials of test and between them and the control group. However, there was a statistically significant difference between different time intervals within each group (P< 0.05) and between different concentration of test materials (P<0.05). In all samples, set materials showed better viability than fresh ones.Conclusion: According to results of this study, NEC and MTA have similar cytotoxic effect on L929 cell culture.

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REVIEW OF ROOT-END FILLING MATERIALS

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APA: Copy

GHODOUSI, JAMILEH, TAVAKOL AFSHARI, J., DONYAVI, Z., BOROUK, A., DISFANI, R., & ESMAEILZADEH, MOHAMMAD. (2008). CYTOTOXIC EFFECT OF A NEW ENDODONTIC CEMENT AND MINERAL TRIOXIDE AGGREGATE ON L929 LINE CULTURE. IRANIAN ENDODONTIC JOURNAL (IEJ), 3(2), 17-23. SID. https://sid.ir/paper/302634/en

Vancouver: Copy

GHODOUSI JAMILEH, TAVAKOL AFSHARI J., DONYAVI Z., BOROUK A., DISFANI R., ESMAEILZADEH MOHAMMAD. CYTOTOXIC EFFECT OF A NEW ENDODONTIC CEMENT AND MINERAL TRIOXIDE AGGREGATE ON L929 LINE CULTURE. IRANIAN ENDODONTIC JOURNAL (IEJ)[Internet]. 2008;3(2):17-23. Available from: https://sid.ir/paper/302634/en

IEEE: Copy

JAMILEH GHODOUSI, J. TAVAKOL AFSHARI, Z. DONYAVI, A. BOROUK, R. DISFANI, and MOHAMMAD ESMAEILZADEH, “CYTOTOXIC EFFECT OF A NEW ENDODONTIC CEMENT AND MINERAL TRIOXIDE AGGREGATE ON L929 LINE CULTURE,” IRANIAN ENDODONTIC JOURNAL (IEJ), vol. 3, no. 2, pp. 17–23, 2008, [Online]. Available: https://sid.ir/paper/302634/en

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