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Information Journal Paper

Title

A NEW METHOD FOR EVALUATING THE DIFFUSION OF CA2+ AND OH- IONS THROUGH CORONAL DENTIN INTO THE PULP

Pages

  189-197

Abstract

 Introduction: Ca (OH)2-containing/forming materials are conventionally used for indirect pulp-capping and are theoretically able to release Ca2+ and OH- ions for hydrolytic dissociation. However, no evidence exists for ion DIFFUSION through the remaining coronal DENTIN. The aim of this study was to design an innovative experimental set-up to test the ability of Ca (OH)2-containing and Ca (OH)2-forming pulp-capping materials to generate pulpward Ca2+and OH- ion fluxes through coronal DENTIN after indirect pulp-capping in vitro.Materials and Methods: Standardized class 1 cavities were prepared in erupted sound human molars. Pulp tissue was excised. A coronal Remaining DENTIN Thickness (RTD) (1±0.2 mm thick) was prepared within an occlusal-to-pulp cavity system (coronal RD system). The whole system/sample was treated with 17% EDTA to remove the smear layer and the external surface was covered by nail varnish. Indirect pulp-capping was performed on coronal RDT using a conventional pulp-capping material covered by a glass ionomer cement, a composite and nail varnish. Chemically different Ca (OH)2 materials were used to test the reliability of the set-up. The leached Ca2+and OH- ions were measured using ion-selective electrodes after soaking for 3 hours, 24 hours, and 7 days in deionized water (10 mL, 37°C).Results: CALCIUM ions were detected and a rise in pH was observed in the treated water after a few hours for all tested materials.Conclusion: The experimental set-up proved to be an easy and effective method for testing the different Ca (OH)2-containing and Ca (OH)2-forming materials ability to induce a pulpward flux of CALCIUM and hydroxyl ions through coronal remaining DENTIN after indirect pulp-capping. The new system will allow the screening of current pulp-capping materials.

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    APA: Copy

    GANDOLFI, MARIA GIOVANNA. (2012). A NEW METHOD FOR EVALUATING THE DIFFUSION OF CA2+ AND OH- IONS THROUGH CORONAL DENTIN INTO THE PULP. IRANIAN ENDODONTIC JOURNAL (IEJ), 7(4), 189-197. SID. https://sid.ir/paper/302853/en

    Vancouver: Copy

    GANDOLFI MARIA GIOVANNA. A NEW METHOD FOR EVALUATING THE DIFFUSION OF CA2+ AND OH- IONS THROUGH CORONAL DENTIN INTO THE PULP. IRANIAN ENDODONTIC JOURNAL (IEJ)[Internet]. 2012;7(4):189-197. Available from: https://sid.ir/paper/302853/en

    IEEE: Copy

    MARIA GIOVANNA GANDOLFI, “A NEW METHOD FOR EVALUATING THE DIFFUSION OF CA2+ AND OH- IONS THROUGH CORONAL DENTIN INTO THE PULP,” IRANIAN ENDODONTIC JOURNAL (IEJ), vol. 7, no. 4, pp. 189–197, 2012, [Online]. Available: https://sid.ir/paper/302853/en

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