THE EFFECTIVENESS OF ORAL REHYDRATION SOLUTION AT VARIOUS CONCENTRATIONS AS A STORAGE MEDIA FOR AVULSED TEETH

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ESKANDARIAN TAHEREH; BADAKHSH SAMANEH; ESMAEILPOUR TAHEREH

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Journal Paper

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Journal: Iranian Endodontic Journal Year:2013 | Volume:8 | Issue:1 Page(s): 22-24

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Information Journal Paper

Title

THE EFFECTIVENESS OF ORAL REHYDRATION SOLUTION AT VARIOUS CONCENTRATIONS AS A STORAGE MEDIA FOR AVULSED TEETH

Author(s)

ESKANDARIAN TAHEREH | BADAKHSH SAMANEH | ESMAEILPOUR TAHEREH | Issue Writer Certificate

Keywords

TOOTH AVULSION

CULTURE MEDIA

REHYDRATION SOLUTIONS

PERIODONTAL LIGAMENT

Abstract

Introduction: Following avulsion, the PERIODONTAL LIGAMENT (PDL) cells are at risk of necrosis. To achieve a favorable survival prognosis, the PDL cells must be kept viability. Therefore, immediate replantation is considered as the treatment of choice and in case it is not possible, storing the tooth in an appropriate storage media should be considered. Oral Rehydration Solution (ORS) is a glucose-electrolyte solution which can keep the optimal osmolality as well as pH and can even provide nutrients which are necessary for cellular growth. The present study aimed to evaluate the effectiveness of different concentrations of ORS in maintaining the viability of the PDL cells at different time points. Materials and Methods: PDL cells were obtained from healthy extracted human premolars. Then, 8×103 cells were seeded in each well of 96-well plate. Afterwards, each well was treated with ORS in three different concentrations and DMEM for 1, 3, 6, and 9 hours. Cell viability was determined by using the MTT assay. One way-ANOVA and post hoc (LSD) test were used for comparing the study groups.Results: According to the results, 25% and 50% concentrations of ORS were more effective in preserving the PDL cell viability and could maintain 79.98% and 68.34% of the PDL cells, respectively, at least for the last experimental time point (up to 9 hours). Conclusions: Therefore, our findings indicate that ORS might be a suitable storage medium for avulsed teeth.

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APA: Copy

ESKANDARIAN, TAHEREH, BADAKHSH, SAMANEH, & ESMAEILPOUR, TAHEREH. (2013). THE EFFECTIVENESS OF ORAL REHYDRATION SOLUTION AT VARIOUS CONCENTRATIONS AS A STORAGE MEDIA FOR AVULSED TEETH. IRANIAN ENDODONTIC JOURNAL (IEJ), 8(1), 22-24. SID. https://sid.ir/paper/302867/en

Vancouver: Copy

ESKANDARIAN TAHEREH, BADAKHSH SAMANEH, ESMAEILPOUR TAHEREH. THE EFFECTIVENESS OF ORAL REHYDRATION SOLUTION AT VARIOUS CONCENTRATIONS AS A STORAGE MEDIA FOR AVULSED TEETH. IRANIAN ENDODONTIC JOURNAL (IEJ)[Internet]. 2013;8(1):22-24. Available from: https://sid.ir/paper/302867/en

IEEE: Copy

TAHEREH ESKANDARIAN, SAMANEH BADAKHSH, and TAHEREH ESMAEILPOUR, “THE EFFECTIVENESS OF ORAL REHYDRATION SOLUTION AT VARIOUS CONCENTRATIONS AS A STORAGE MEDIA FOR AVULSED TEETH,” IRANIAN ENDODONTIC JOURNAL (IEJ), vol. 8, no. 1, pp. 22–24, 2013, [Online]. Available: https://sid.ir/paper/302867/en

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