Effect of Different Additives on Genotoxicity of Mineral Trioxide Aggregate

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SAMIEI MOHAMMAD; SHAHI SHAHRIAR; GHASEMI NEGIN; DASTMALCHI SIAVOUSH; BARGAHI NASRIN; ASGARY SAEED

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Journal: Iranian Endodontic Journal Year:2018 | Volume:13 | Issue:1 Page(s): 37-41

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Title

Effect of Different Additives on Genotoxicity of Mineral Trioxide Aggregate

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Keywords

Ames Test

Disodium Hydrogen Phosphate

Genotoxicity

Mineral Trioxide Aggregate

Nano Silver

Abstract

Introduction: The aim of the present in vitro study was to evaluate the Genotoxicity of Mineral Trioxide Aggregate (MTA) after adding different concentrations of Disodium Hydrogen Phosphate and silver nanoparticles using the Ames Test. Methods and Materials: TA100 strain of Salmonella typhimurium was used to evaluate mutagenicity of experimental materials with and without S9 mix fraction. The materials tested in this study consisted of MTA, MTA/Disodium Hydrogen Phosphate and MTA/silver nanoparticles at 0. 1, 0. 01, 0. 001 and 0. 0001 concentrations. Negative and positive control groups consisted of 1% dimethyl sulfoxide and sodium azide with 2-aminoanthracene, respectively. The number of colonies per plate was determined. If the ratio of the number of histidine-revertant colonies to spontaneous revertants of the negative control colonies was *2, the material was regarded a mutagenic agent. Results: In all the concentrations of the three tested materials, the Ames Test failed to detect mutations. Conclusion: Under the limitations of the present study, MTA/Disodium Hydrogen Phosphate and MTA/silver nanoparticles were biocompatible in relation to mutagenicity.

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APA: Copy

SAMIEI, MOHAMMAD, SHAHI, SHAHRIAR, GHASEMI, NEGIN, DASTMALCHI, SIAVOUSH, BARGAHI, NASRIN, & ASGARY, SAEED. (2018). Effect of Different Additives on Genotoxicity of Mineral Trioxide Aggregate. IRANIAN ENDODONTIC JOURNAL (IEJ), 13(1), 37-41. SID. https://sid.ir/paper/303031/en

Vancouver: Copy

SAMIEI MOHAMMAD, SHAHI SHAHRIAR, GHASEMI NEGIN, DASTMALCHI SIAVOUSH, BARGAHI NASRIN, ASGARY SAEED. Effect of Different Additives on Genotoxicity of Mineral Trioxide Aggregate. IRANIAN ENDODONTIC JOURNAL (IEJ)[Internet]. 2018;13(1):37-41. Available from: https://sid.ir/paper/303031/en

IEEE: Copy

MOHAMMAD SAMIEI, SHAHRIAR SHAHI, NEGIN GHASEMI, SIAVOUSH DASTMALCHI, NASRIN BARGAHI, and SAEED ASGARY, “Effect of Different Additives on Genotoxicity of Mineral Trioxide Aggregate,” IRANIAN ENDODONTIC JOURNAL (IEJ), vol. 13, no. 1, pp. 37–41, 2018, [Online]. Available: https://sid.ir/paper/303031/en

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