CLONING AND SEQUENCING OF LEISHMANIA MAJOR THIOL-SPECIFIC-ANTIOXIDANT ANTIGEN (TSA) GENE

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

TABATABAEI F.; GHAFARIFAR F.; DALIMI A.; SHARIFI Z.; ZAVARAN HOSSEINI A.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF PARASITOLOGY Year:2007 | Volume:2 | Issue:4 Page(s): 30-41

Download Full-Text

View:

433

Download:

149

Cites:

Information Journal Paper

Title

CLONING AND SEQUENCING OF LEISHMANIA MAJOR THIOL-SPECIFIC-ANTIOXIDANT ANTIGEN (TSA) GENE

Author(s)

Keywords

CLONING

SEQUENCING

LEISHMANIA MAJOR

TSA

Abstract

Background: Leishmaniasis is caused by parasitic protozoa of the genus Leishmania which, in the infected host are obligate intracellular parasite. TSA is the immuno-dominant antigen of LEISHMANIA MAJOR which is considered as the most promising molecule for a recombinant or DNA vaccine against leishmaniasis.Methods: Genomic DNA of TSA protein was extracted and amplificated as a template. Then the PCR product was cloned into pTZ57R/T vector. Finally, the recombinant plasmid was extracted from transformed Escherichia coli (TG1 strain) and sequenced.Results: MRHO/IR/75/ER (an Iranian strain) of L. major and TSA gene (Accession number LmjF15.1080) were used. Sequence analysis of cloned TSA gene into pTZ57R/T vector showed high homology of 90% with LmjF15.1080 (TSA gene) and strain "LV39" (Accession no. AF069386) and strain "Friedlin" (Accession no.AF044679).Conclusion: We cloned TSA gene of L. major successfully. Recombinant plasmid was confirmed. It is ready to express recombinant protein for further studies.

Cites

No record.

References

No record.

Cite

APA: Copy

TABATABAEI, F., GHAFARIFAR, F., DALIMI, A., SHARIFI, Z., & ZAVARAN HOSSEINI, A.. (2007). CLONING AND SEQUENCING OF LEISHMANIA MAJOR THIOL-SPECIFIC-ANTIOXIDANT ANTIGEN (TSA) GENE. IRANIAN JOURNAL OF PARASITOLOGY, 2(4), 30-41. SID. https://sid.ir/paper/303792/en

Vancouver: Copy

TABATABAEI F., GHAFARIFAR F., DALIMI A., SHARIFI Z., ZAVARAN HOSSEINI A.. CLONING AND SEQUENCING OF LEISHMANIA MAJOR THIOL-SPECIFIC-ANTIOXIDANT ANTIGEN (TSA) GENE. IRANIAN JOURNAL OF PARASITOLOGY[Internet]. 2007;2(4):30-41. Available from: https://sid.ir/paper/303792/en

IEEE: Copy

F. TABATABAEI, F. GHAFARIFAR, A. DALIMI, Z. SHARIFI, and A. ZAVARAN HOSSEINI, “CLONING AND SEQUENCING OF LEISHMANIA MAJOR THIOL-SPECIFIC-ANTIOXIDANT ANTIGEN (TSA) GENE,” IRANIAN JOURNAL OF PARASITOLOGY, vol. 2, no. 4, pp. 30–41, 2007, [Online]. Available: https://sid.ir/paper/303792/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops