ISOLATION OF DNA FROM A SINGLE HELMINTH USING NEW DEVELOPED KIT IN IRAN AND ITS PCR ANALYSIS

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SHAYAN P.; BORJI H.; ESLAMI ALI; ZAKERI S.

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Journal Paper

Paper Information

Journal: Iranian Journal of Parasitology Year:2007 | Volume:2 | Issue:2 Page(s): 34-39

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Information Journal Paper

Title

ISOLATION OF DNA FROM A SINGLE HELMINTH USING NEW DEVELOPED KIT IN IRAN AND ITS PCR ANALYSIS

Author(s)

SHAYAN P. | BORJI H. | ESLAMI ALI | ZAKERI S. | Issue Writer Certificate

Keywords

GENOMIC DNA

PCR

SEMI-NESTED PCR

IRAN

Abstract

Background: Nematodes are among the most common and important parasites of man and animal. DNA of a single worm can be used for several purposes, such as identification to the species, subspecies, strain and antihelmenthic resistance. DNA extraction from a single small worm using traditional methods such as phenol extraction technique faces serious problems.Methods: DNA from 20 single Haemonchus contortus was isolated using DNA isolation kit newly designed in IRAN by the Research Unit of Molecular Biological System Transfer (MBST) based on the specific binding of DNA to the carrier. The GENOMIC DNA was amplified using specific primers derived from b -tubulin isotype 1 in PCR. The specificity of the PCR products was determined using semi-nested PCR technique. Specific PCR-product from b-tubulin gene could be amplified with 1ng, 100pg and 10pg DNA.Results: The used DNA extraction method was safe, with high quality and quantity, fast, easy to handle and not costly for genetic analysis of even a single small worm.Conclusion: The IRAN produced DNA extraction Kit is grounded on a selective binding of nucleic acids to a silica-based membrane and is recommended for the isolation of DNA from even small amount of biological materials.

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APA: Copy

SHAYAN, P., BORJI, H., ESLAMI, ALI, & ZAKERI, S.. (2007). ISOLATION OF DNA FROM A SINGLE HELMINTH USING NEW DEVELOPED KIT IN IRAN AND ITS PCR ANALYSIS. IRANIAN JOURNAL OF PARASITOLOGY, 2(2), 34-39. SID. https://sid.ir/paper/303819/en

Vancouver: Copy

SHAYAN P., BORJI H., ESLAMI ALI, ZAKERI S.. ISOLATION OF DNA FROM A SINGLE HELMINTH USING NEW DEVELOPED KIT IN IRAN AND ITS PCR ANALYSIS. IRANIAN JOURNAL OF PARASITOLOGY[Internet]. 2007;2(2):34-39. Available from: https://sid.ir/paper/303819/en

IEEE: Copy

P. SHAYAN, H. BORJI, ALI ESLAMI, and S. ZAKERI, “ISOLATION OF DNA FROM A SINGLE HELMINTH USING NEW DEVELOPED KIT IN IRAN AND ITS PCR ANALYSIS,” IRANIAN JOURNAL OF PARASITOLOGY, vol. 2, no. 2, pp. 34–39, 2007, [Online]. Available: https://sid.ir/paper/303819/en

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