SUBCLONING AND EXPRESSION OF RECOMBINANT ECHINOCOCCUS GRANULOSUS ANTIGEN B, IN PQE-30 EXPRESSION VECTOR

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

TAGHIPOUR N.; BANDEHPOUR M.; PAZOKI R.; HAGHIGHI A.; NAZARI POUYA M.R.; KAZEMI B.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF PARASITOLOGY Year:2009 | Volume:4 | Issue:4 Page(s): 1-9

Download Full-Text

View:

392

Download:

223

Cites:

Information Journal Paper

Title

SUBCLONING AND EXPRESSION OF RECOMBINANT ECHINOCOCCUS GRANULOSUS ANTIGEN B, IN PQE-30 EXPRESSION VECTOR

Author(s)

Keywords

CYSTIC ECHINOCOCCOSIS

RECOMBINANT ANTIGEN B

HI GENE

Abstract

Background: Echinococcosis or hydatid disease is a zoonotic infection caused by larval (metacestode) stages of cestodes belonging to the genus Echinococcus, family Taeniidae. We aimed to subclone antigen B gene in pQE-30 plasmid, its expression, and purification. Methods: We subcloned HI GENE into pQE-30 expression vector. The recombinant vector was transformed into E. coli, M15 and mass cultured. The subcloned gene was expressed by IPTG. Subcloning of gene was confirmed by both PCR and enzyme digestion.Results: Production of recombinant protein was confirmed by SDS-PAGE. Western blot analysis was carried out by both His-Tag monoclonal Ab and human serum to estimate the expressed protein in E. coli cells. Recombinant protein was purified and its specificity was proved by Western blotting.Conclusion: Production of this recombinant protein can increase sensitivity and specificity in serological test (ELISA).

Cites

No record.

References

No record.

Cite

APA: Copy

TAGHIPOUR, N., BANDEHPOUR, M., PAZOKI, R., HAGHIGHI, A., NAZARI POUYA, M.R., & KAZEMI, B.. (2009). SUBCLONING AND EXPRESSION OF RECOMBINANT ECHINOCOCCUS GRANULOSUS ANTIGEN B, IN PQE-30 EXPRESSION VECTOR. IRANIAN JOURNAL OF PARASITOLOGY, 4(4), 1-9. SID. https://sid.ir/paper/303888/en

Vancouver: Copy

TAGHIPOUR N., BANDEHPOUR M., PAZOKI R., HAGHIGHI A., NAZARI POUYA M.R., KAZEMI B.. SUBCLONING AND EXPRESSION OF RECOMBINANT ECHINOCOCCUS GRANULOSUS ANTIGEN B, IN PQE-30 EXPRESSION VECTOR. IRANIAN JOURNAL OF PARASITOLOGY[Internet]. 2009;4(4):1-9. Available from: https://sid.ir/paper/303888/en

IEEE: Copy

N. TAGHIPOUR, M. BANDEHPOUR, R. PAZOKI, A. HAGHIGHI, M.R. NAZARI POUYA, and B. KAZEMI, “SUBCLONING AND EXPRESSION OF RECOMBINANT ECHINOCOCCUS GRANULOSUS ANTIGEN B, IN PQE-30 EXPRESSION VECTOR,” IRANIAN JOURNAL OF PARASITOLOGY, vol. 4, no. 4, pp. 1–9, 2009, [Online]. Available: https://sid.ir/paper/303888/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops