WHATMAN PAPER (FTA CARDS) FOR STORING AND TRANSFERRING LEISHMANIA DNA FOR PCR EXAMINATION

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FATA A.; KHAMESIPOUR A.; MOHAJERI M.; HOSSEININEZHAD Z.; AFZAL AGHAEI M.; BERENJI F.; GANJBAKHSH M.; AKHAVAN A.A.; ESKANDARI E.; AMIN MOHAMMADI A.

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Journal Paper

Paper Information

Journal: Iranian Journal of Parasitology Year:2009 | Volume:4 | Issue:4 Page(s): 37-42

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Title

WHATMAN PAPER (FTA CARDS) FOR STORING AND TRANSFERRING LEISHMANIA DNA FOR PCR EXAMINATION

Author(s)

Keywords

LEISHMANIASIS

PCR

WHATMAN FILTER PAPER

Abstract

Background: Diagnosis of cutaneous LEISHMANIASIS (CL) is often made based on clinical manifestation. Correct diagnosis and identification of the parasite are crucial for choosing the effective treatment and for epidemiological studies. On the other hand, determination of Leishmania species is necessary for designing appropriate control programs. Diagnosis by PCR is becoming a 'gold standard'. For PCR preparation, storage and shipments of specimens are necessary. In this study, WHATMAN FILTER PAPER (FTA Card) was used to store and transfer samples for Leishmania identification using PCR. Methods: Among the patients who had CL lesion and referred to Parasitology Laboratory of Emam Reza Hospital, Mashhad, Iran, 44 consented cases with positive results in their direct smear were selected. An informed consent form and a questionnaire were completed and three different types of samples (direct smear, NNN culture, and spot on FTA card) were collected. DNA extraction and PCR were carried out on three different samples from each patient.Results: PCR results using Whatman paper samples revealed a significant difference (P<0.0001) compared to the culture method but no significant difference was seen between PCR results using samples stored on Whatman paper and direct smears. Conclusion: The use of FTA cards is simple, rapid, and cost-effective, and can be readily employed for large-scale population screening, especially for regions where the specimens are to be transported from distant places to the laboratory.

Cites

CORRELATION BETWEEN THE RESULT OF LEISHMANIN SKIN TEST AND CLINICAL FORMS OF CUTANEOUS LEISHMANIASIS AND SPECIES OF PARASITE

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APA: Copy

FATA, A., KHAMESIPOUR, A., MOHAJERI, M., HOSSEININEZHAD, Z., AFZAL AGHAEI, M., BERENJI, F., GANJBAKHSH, M., AKHAVAN, A.A., ESKANDARI, E., & AMIN MOHAMMADI, A.. (2009). WHATMAN PAPER (FTA CARDS) FOR STORING AND TRANSFERRING LEISHMANIA DNA FOR PCR EXAMINATION. IRANIAN JOURNAL OF PARASITOLOGY, 4(4), 37-42. SID. https://sid.ir/paper/303895/en

Vancouver: Copy

FATA A., KHAMESIPOUR A., MOHAJERI M., HOSSEININEZHAD Z., AFZAL AGHAEI M., BERENJI F., GANJBAKHSH M., AKHAVAN A.A., ESKANDARI E., AMIN MOHAMMADI A.. WHATMAN PAPER (FTA CARDS) FOR STORING AND TRANSFERRING LEISHMANIA DNA FOR PCR EXAMINATION. IRANIAN JOURNAL OF PARASITOLOGY[Internet]. 2009;4(4):37-42. Available from: https://sid.ir/paper/303895/en

IEEE: Copy

A. FATA, A. KHAMESIPOUR, M. MOHAJERI, Z. HOSSEININEZHAD, M. AFZAL AGHAEI, F. BERENJI, M. GANJBAKHSH, A.A. AKHAVAN, E. ESKANDARI, and A. AMIN MOHAMMADI, “WHATMAN PAPER (FTA CARDS) FOR STORING AND TRANSFERRING LEISHMANIA DNA FOR PCR EXAMINATION,” IRANIAN JOURNAL OF PARASITOLOGY, vol. 4, no. 4, pp. 37–42, 2009, [Online]. Available: https://sid.ir/paper/303895/en

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