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Information Journal Paper

Title

RAPID DETECTION OF TOXOPLASMA GONDII ANTIGEN IN EXPERIMENTALLY INFECTED MICE BY DOT- ELISA

Pages

  28-33

Abstract

 Background: Toxoplasmosis is a worldwide endemic disease. In congenitally infected infants and AIDS patients, toxoplasmosis causes high rates of morbidity and mortality. In these cases antibody detection is difficult; so detection of parasite or its components could be useful tool for early detection and following treatment of the infection.Methods: Sixty-three BALB/c mice were injected intra-peritoneal with 5×103 tachyzoites of TOXOPLASMA GONDIIRH strain, nine mice were sacrificed daily for 7 days. Fourteen mice were injected with phosphate buffer saline as control group. Dot–ELISA was performed for detection of T.gondiiantigen in mice sera and CAPTURE-ELISA was done as golden standard assay too.Results: TOXOPLASMA GONDII antigen was detected from day 2 in mice sera; 22% of mice sera on day 2, 33% on day 3, 77% on day 4 and 100% on day 5 till their death on day 7 had shown ANTIGENEMIA by DOT-ELISA, no positive result was detected in control mice by dot- ELISA.Conclusion: DOT-ELISA is a sensitive method for diagnosis of T. gondii infection in the animal model; also, this technique is more rapid and easy to perform method in comparison with CAPTURE-ELISA.

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    APA: Copy

    JAFAR POUR AZAMI, S., KESHAVARZ, H., REZAIAN, M., MOHEBALI, M., & SHOJAEE, S.. (2011). RAPID DETECTION OF TOXOPLASMA GONDII ANTIGEN IN EXPERIMENTALLY INFECTED MICE BY DOT- ELISA. IRANIAN JOURNAL OF PARASITOLOGY, 6(1), 28-33. SID. https://sid.ir/paper/303948/en

    Vancouver: Copy

    JAFAR POUR AZAMI S., KESHAVARZ H., REZAIAN M., MOHEBALI M., SHOJAEE S.. RAPID DETECTION OF TOXOPLASMA GONDII ANTIGEN IN EXPERIMENTALLY INFECTED MICE BY DOT- ELISA. IRANIAN JOURNAL OF PARASITOLOGY[Internet]. 2011;6(1):28-33. Available from: https://sid.ir/paper/303948/en

    IEEE: Copy

    S. JAFAR POUR AZAMI, H. KESHAVARZ, M. REZAIAN, M. MOHEBALI, and S. SHOJAEE, “RAPID DETECTION OF TOXOPLASMA GONDII ANTIGEN IN EXPERIMENTALLY INFECTED MICE BY DOT- ELISA,” IRANIAN JOURNAL OF PARASITOLOGY, vol. 6, no. 1, pp. 28–33, 2011, [Online]. Available: https://sid.ir/paper/303948/en

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